Total Exosome Isolation Reagent (from urine) enables fast and efficient enrichment of intact exosomes from urine samples with a protocol that is scalable depending on your sample size.

• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—can be scaled up or down depending on the sample size

By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the urine, and the solution is incubated for 1 hour at room temperature. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 1 hour at 2–8°C. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.

For downstream mass spectrometry analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.