The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.
• Generalized mechanism allows for measurement of a variety of proteases • Can be used to continuously measure protease kinetics • Excitation/emission similar to Texas Red™ dye (589/617 nm)
This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY™ TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY™ TR-X dye has excitation and emission spectra similar to those of the Texas Red™ fluorophore.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Fluorescence Intensity
For Use With (Application)
Protease Assay
For Use With (Equipment)
Fluorescence Microplate Reader
Product Type
Protease Assay
Product Line
EnzChek™
Shipping Condition
Room Temperature
Substrate Properties
Protein-Based Substrate
Substrate Type
Protease Substrate
Target Enzyme
Proteases
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.