The ViraPower™ Lentiviral Directional TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO™ technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO™ vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.
Advantages • Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells
Key Features • Quick convenient Directional TOPO™ cloning technology • Constitutive high expression with CMV promoter • Blasticidin selection marker for stable selection • C terminal V5 tag for quick detection
Kit includes • pLenti6⁄V5-D™-TOPO™ vector • ViraPower™ Bsd Lentiviral Support Kit (K4970-00) • 293FT Cell Line (R70007) • One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)