The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity
100
Format
Tube
Product Type
Transition Pore Assay Kit
Excitation/Emission
488
Shipping Condition
Room Temperature
Product Line
MitoProbe™
Conjugate
Calcein AM
Contents & Storage
Contains 5 vials of calcein, AM (50 μg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 μg), calcium salt, and 1 vial of DMSO (1.5 mL).