FreeStyle™ CHO Expression Medium (Gibco®)

FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. ...

Specifications

Form
Liquid
Format
Bottle(s)
Cell Line
CHO
FreeStyle™ CHO-S Cells
Glutamine
No Glutamine
Serum Level
Serum-Free
Culture Type
Suspension Cell Culture
Classification
Serum-Free
Protein-Free
Chemically Defined
Endotoxin Level
≤ 0.25 EU/ml
Phenol Red Indicator
No Phenol Red
Green Features
Sustainable packaging
Shipping Condition
Room Temperature
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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FreeStyle™ CHO-S Cells (Invitrogen™)

FreeStyle™ CHO-S Cells are part of the FreeStyle™ MAX Expression System, which is a breakthrough technology for rapid and high-yield mammalian protein production. This cell line is adapted to high density, serum-free suspension culture in FreeStyle™ CHO Expression Medium and is c ...

Specifications

Species
Hamster
Cell Line
FreeStyle™ CHO-S Cells
System
FreeStyle™
Number of Cells
1 x 107

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FreeStyle™ MAX CHO Expression System (Invitrogen™)

The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect Free ...

Specifications

Cell Line
FreeStyle™ CHO-S Cells
Expression
Mammalian
Culture Type
Suspension Cell Culture
Key Function
Protein Production
Classification
Animal Origin-Free
Serum-Free
Delivery Method
Transfection
Expression Mechanism
Cell-Based Expression
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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FreeStyle™ MAX Reagent (Invitrogen™)

FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection r ...

Specifications

Cell Type
Established Cell Lines
Sample Type (Specific)
Plasmid DNA
Classification
Animal Origin-Free
Transfection Technique
Lipid-Based Transfection
Serum Compatible
Yes
Shipping Condition
Wet Ice
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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FreeStyle™ F17 Expression Medium (Gibco®)

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of tra ...

Specifications

Form
Liquid
Format
Universal Bag(s)
Cell Line
FreeStyle™ 293-F Cells
Glutamine
No Glutamine
Sterility
Sterile
Serum Level
Serum-Free
Supplements
2-mercaptoethanol
Concentrated
1 X
Culture Type
Suspension Cell Culture
Classification
Animal Origin-Free
Protein-Free
Serum-Free
Chemically Defined
Endotoxin Level
< 1 EU/ml
Culture Environment
CO2
Purity or Quality Grade
Qualified for Further Manufacturing
Shipping Condition
Room Temperature

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pcDNA™3.4 TOPO® TA Cloning Kit (Invitrogen™)

The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expr ...

Specifications

Promoter
CMV
Key Function
Protein Production
Constitutive Expression
Protein Tag or Fusion
Untagged
Delivery Method
Transfection
Cloning Method
TOPO®-TA
Selection Agent (Eukaryotic)
Geneticin® (G-418)
Vector Type
pcDNA
Structure
Linear
Plasmid Type
High Copy Plasmid
Special Elements
WPRE
Replication Origin
pUC Origin
Selection Marker Promoter
SV40 Promoter
Selection Marker (Eukaryotic)
NeoR
Constitutive or Inducible System
Constitutive
Antibiotic Resistance (Bacterial)
Ampicillin (AmpR)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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