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    [24]

The role of template-primer in protection of reverse transcriptase from thermal inactivation.

Citations & References

  • Authors: Gerard GF, Potter RJ, Smith MD, Rosenthal K, Dhariwal G, Lee J, Chatterjee DK,
  • Journal: Nucleic Acids Res (2002) 30:3118-3129
  • Product Usage: Comparison of thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity.
  • ID: PN65017
Catalog #s
c2c1fc225872c8f4b8b18410e10ff072_Citations

Ethanol hypersensitivity and olfactory discrimination defect in mice lacking a homolog of Drosophila neuralized.

Citations & References

  • Authors: Ruan Y; Tecott L; Jiang M M; Jan L Y; Jan Y N;
  • Journal: Proc Natl Acad Sci U S A (2001) 98:9907-12
  • Product Usage: Total RNA was isolated from a series of embryonic stages of mouse embryos. Oligo(dT)-primed reverse transcription was performed on 2.0 µg of total RNA from each embryonic stage with M-MLV reverse transcriptase.
  • ID: PN65613
Catalog #s
0
df8a6aa94e8b9dafafb2761be5c197c5_Citations

A DEAD-Box Protein Functions as an ATP-Dependent RNA Chaperone in Group I Intron Splicing.

Citations & References

  • Authors: Mohr Sabine; Stryker John M; Lambowitz Alan M;
  • Journal: Cell (2002) 109:769-79
  • Product Usage: The PCR product was labeled with [ -32P]dCTP (3000 Ci/mmol) by random priming using a RadPrime kit. Modification sites were mapped by primer extension with M-MLV RT, using 5'-labeled primers complementary to different regions of the intron RNA.
  • ID: PN63254
Catalog #s
18428011
f3717533a90a8ecb1de730050beab052_Citations

Blockade of the intermediate-conductance calcium-activated potassium channel as a new therapeutic strategy for restenosis.

Citations & References

  • Authors: Köhler R, Wulff H, Eichler I, Kneifel M, Neumann D, Knorr A, Grgic I, Kämpfe D, Si H, Wibawa J, Real R, Borner K, Brakemeier S, Orzechowski HD, Reusch HP, Paul M, Chandy KG, Hoyer J,
  • Journal: Circulation (2003) 108:1119-1125
  • Product Usage: Total RNA was isloated from rat Vascular smooth muscle cells(VSMC)with TRIzol reagent and revesed transcribed with M-MLV Reverse Transcriptase prior to quantitative real-time PCR.
  • ID: PN63028
Catalog #s
f47a07182433c042d84cb754ddcac64f_Citations

A functional role for Tsix transcription in blocking Xist RNA accumulation but not in X-chromosome choice.

Citations & References

  • Authors: Stavropoulos N; Lu N; Lee J T;
  • Journal: Proc Natl Acad Sci U S A (2001) 98:10232-10239
  • Product Usage: For allele-specific RT-PCR, RNA was isolated with Trizol , DNase treated , and reverse transcribed M-MLV RT and 200 ng of random primer, or at 50°C with Superscript II and 3 pmol of strand-specific primer.
  • ID: PN61968
Catalog #s
15596026
18064105
5c9d54bf8c2ade6c7ad0bafd578e5f09_Citations

XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor.

Citations & References

  • Authors: Yoshida H; Matsui T; Yamamoto A; Okada T; Mori K;
  • Journal: Cell (2001) 107:881-972
  • Product Usage: A portion of the 5' region missing in the cDNA was isolated by the rapid amplification of cDNA ends method using HeLa cell RNA and 5'-RACE System (Invitrogen). The entire XBP1 cDNA obtained was inserted into pcDNA3.1(+) (Invitrogen) to create pcDNA-XBP1(unspliced). Aliquots of 20 µg of total RNA were treated with M-MLV reverse transcriptase (Invitrogen) and then amplified.
  • ID: PN65298
Catalog #s
18064022
V79020
02c988e1e2c701afc4dae1df08630f87_Citations

Intracellular expression of the plasmid-encoded toxin from enteroaggregative Escherichia coli.

Citations & References

  • Authors: Sui BQ, Dutta PR, Nataro JP,
  • Journal: Infect Immun (2003) 71:5364-5370
  • Product Usage: For construction of pcDNA3.1-pet and pcDNA3.1-S260A were amplified by PCR and cloned into pcDNA3.1 cut with NheI and XhoI. Total RNA was isolated from cells transfected by pcDNA3.1, and above constructs using Trizol. 1 Kb-Plus DNA Ladder for sizing of DNA. RT was performed with M-MLV reverse transcriptase. HEp-2 cells were propagated in humidified 5% CO2 at 37°C in DMEM with 10% FBS. Transfection was performed with 4 ug of plasmid and 40 ug of Lipofectin added to 300 ul of serum-free DMEM.
  • ID: PN61917
Catalog #s
60b4f063604e40c044bb1113825bc17a_Citations

Heart regeneration in adult MRL mice.

Citations & References

  • Authors: Leferovich J M; Bedelbaeva K; Samulewicz S; Zhang X M; Zwas D; Lankford E B; Heber-Katz E;
  • Journal: Proc Natl Acad Sci U S A (2001) 98:9830-5
  • Product Usage: Dissected hearts were ground to a fine powder in liquid nitrogen. RNA was isolated by dissolving the powder in Trizol. Two micrograms of total RNA were reverse transcribed in 20 µl of mixture that included 10 units of RNaseIN, 0.2 µM dNTP, 1 µM random hexamer, and 200 units of M-MLV reverse transcriptase.
  • ID: PN62943
Catalog #s
15596026
d644c7cbbb1064491c3b1334c99b25c8_Citations

The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnover.

Citations & References

  • Authors: Toompuu Marina; Yasukawa Takehiro; Suzuki Tsutomu; Hakkinen Terhi; Spelbrink Johannes N; Watanabe Kimitsuna; Jacobs Howard T;
  • Journal: J Biol Chem (2002) 277:22240-50
  • Product Usage: total RNA were extracted by Trizol extraction (Invitrogen). RT-PCR used 0.0125 A260 units of random hexamers and Invitrogen M-MLV-RTase cloned into BamHI/HindIII-digested pcDNA3.1(-) Myc/His A (Invitrogen) 4 µg of DNA and 40 µg of LipofectAMINE (Invitrogen) were diluted in 4 ml of Opti-MEM (Invitrogen) and added to cells washed with Opti-MEM. after 24 h cells were placed under selection in 1.6 mg/ml Geneticin (Invitrogen).
  • ID: PN60086
Catalog #s
15596026
V80020
48ea3e6f887cf74cbff9dd2a13d81bd2_Citations

A monoclonal cytolytic T-lymphocyte response observed in a melanoma patient vaccinated with a tumor-specific antigenic peptide encoded by gene MAGE-3.

Citations & References

  • Authors: Coulie P G; Karanikas V; Colau D; Lurquin C; Landry C; Marchand M; Dorval T; Brichard V; Boon T;
  • Journal: Proc Natl Acad Sci U S A (2001) 98:10290-5
  • Product Usage: Total RNA from PBMC or tumor material was extracted and converted to cDNA with M-MLV reverse transcriptase.
  • ID: PN64430
Catalog #s
0
4ab35559cc92240547c18e228f234484_Citations

Identification and functional analysis of the rat caspase-3 gene promoter.

Citations & References

  • Authors: Liu Wenfang; Wang Geping; Yakovlev Alexander G;
  • Journal: J Biol Chem (2002) 277:8273-8281
  • Product Usage: DNA from a PCR-positive P1 clone was isolated, digested with BamHI or HindIII restriction endonucleases, and subcloned in pZErO-2 plasmid vector. After 5' RACE, the longest detected PCR products were then cloned in the pCR2.1 TA-cloning vector. Cellular RNA was isolated by acidic phenol extraction and 5 µg was reverse transcribed with M-MLV RT.
  • ID: PN65312
Catalog #s
K203040
K260001
4526ff9c12cbeaf09fff0a778938b52e_Citations

Neuropeptide AF and FF modulation of adipocyte metabolism. Primary insights from functional genomics and effects on beta-adrenergic responsiveness.

Citations & References

  • Authors: Lefrere I, De Coppet P, Camelin JC, Le Lay S, Mercier N, Elshourbagy N, Bril A, Berrebi-Bertrand I, Feve B, Krief S
  • Journal: J Biol Chem (2002) 277:39169-78
  • Product Usage: cDNA was synthesized from 5 µg of total RNA using random hexamers and M-MLV reverse transcriptase. 3T3-L1 cells were grown and differentiated at 37 °C in an atmosphere of air/CO2 in DMEM with 4.5 g/liter of D-glucose, 10% fetal calf serum, penicillin/streptomycin (50 units of penicillin/50 µg of streptomycin/ml of medium). After 10 days, cells were placed for 16–18 h in a defined medium consisting of DMEM/Ham’s F-12, 4.5 g/liter of D-glucose, L-glutamine, penicillin/streptomycin, 5% BSA
  • ID: PN47907
Catalog #s
11965092
15140031
21700018
S7563
S7567
S7580
S7585
3d2d30960eae9770a232641d29158db0_Citations

Plasminogen Activator Inhibitor-1 and -3 Increase Cell Adhesion and Motility of MDA-MB-435 Breast Cancer Cells.

Citations & References

  • Authors: Palmieri Diane; Lee Jung Weon; Juliano Rudy L.; Church Frank C.;
  • Journal: J Biol Chem (2002) 277:40950-40957
  • Product Usage: The gene of the Plasminogen activator inhibitor-1 (PAI-1), linked to breast cancer is studied. M-MLV was used for RT RNA from MDA-MB-435 and MDA-MB-231 breast tumor cells and the hepatocellular carcinoma cell line Hep G2 ; and the RT/PCR products were cloned into pcDNA3.1.
  • ID: PN48042
Catalog #s
F432
H1398
H21491
H3569
V79020
d82c8d1619ad8176d665453cfb2e55f0_Citations

Evidence for Hox and E2A-PBX1 collaboration in mouse T-cell leukemia

Citations & References

  • Authors: Bijl, J; Krosl, J; Lebert-Ghali, CE; Vacher, J; Mayotte, N; Sauvageau, G
  • Journal: ONCOGENE 2008 49:6356-6364
  • Product Usage: SYBR® Green Reagents
  • ID: PN96569
Catalog #s
a9cc20a43aa3bf08ad79d2e658e4da26_Citations

Expression analysis of genes involved in brain tumor progression driven by retroviral insertional mutagenesis in mice.

Citations & References

  • Authors: Johansson FK; Goransson H; Westermark B
  • Journal: Oncogene 2005 24:3896-3905
  • Product Usage: SYBR® Green Reagents
  • ID: PN70569
Catalog #s
348da8d53eef840b25d08da3f236972c_Citations