Z′-LYTE® Kinase Assay Kit - Ser/Thr 17 Peptide

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Catalog # Unit Size List Price
PV4316 800 x 20 µL assays 530.00

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing   View full product details >

General Specifications

Format
384-well plate
Readout
End Point
Technique
FRET
Assay Type
Serine⁄Threonine Kinase Assay
References
Biochem J. vol 296, pp. 843-9 (1993)
Label or Dye
Coumarin
Product Size
800 x 20 µL assays
Target Enzyme
Serine⁄Threonine Kinases
Validated Kinases
CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2
Number of Reactions
800 Reactions
Substrate Properties
Peptide-Based Substrate
Protein or Peptide Source
similar to Ser-7 of glycogen synthase KKLNRTLSVA (KKLNRTLSFAEPG)
Excitation⁄Emission (nm)
405⁄465, 535
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Product Applications

  • Drug Discovery & Development
  • Proteins, Expression, Isolation & Analysis
PV4316
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Fluorescein-CREBtide Substrate

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Catalog # Unit Size List Price
PV3508 1 mg 668.00

Description

A fluorescein-labeled peptide substrate that is based upon the CREB ( cAMP responsive element binding) protein. The peptide substrate is phosphorylated by a wide range of Ser/Thr   View full product details >

General Specifications

Substrate
Fluorescein-CREBtide
Label or Dye
FITC (Fluorescein)
Product Size
1 mg
Target Enzyme
Serine⁄Threonine Kinases
Substrate Type
Kinase Substrate
Detection Method
Fluorescent
Molecular Weight
2076 Da
Validated Kinases
AMPK, AURKA, CAMK1, CAMKID, CAMK2A, CAMK2B, CAMK4, CHEK1, CHEK2, MAPKAPK2, MAPKAPK3, PASK, PIM1, PIM2, PKN1, PRKACA, PRKD1, PRKD2, PRKG1, PRKG2, RPS6KA4, RPS6KA5, STK22D
Amino Acid Sequence
Fluorescein- KRREILSRRPSYR
Substrate Properties
Peptide-Based Substrate
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Product Applications

  • Drug Discovery & Development
  • Proteins, Expression, Isolation & Analysis
PV3508
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Omnia® S/T Peptide 8 Kit

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Catalog # Unit Size List Price
KNZ1081 1 kit 930.00

Description

The Omnia® kinase assay utilizes peptide substrates incorporating the proprietary SOX moiety to measure phosphorylation in an antibody-free format. This enables the user to   View full product details >

General Specifications

Form
Frozen
Color
Green
Readout
Kinetic
Platform
Microplate Reader
Technique
Fluorescence Intensity
Assay Type
Serine⁄Threonine Kinase Assay
References
Nature Methods Vol 2 pps 277-283 (2005) and JACS Vol 125 pps 14248 - 14249 (2003)
Label or Dye
Sox Chelation-Enhanced Fluorophore
Product Size
1 kit
Target Enzyme
Serine/Threonine Kinases
Substrate Type
Kinase Substrate
Detection Method
Fluorescent
Molecular Weight
1237 Da
Validated Kinases
MAP3K11 (MLK3), MAP3K9 (MLK1), MINK1, PRKD2 (Protein Kinase D2), PRKD1 (PKC mu), PKC
Amino Acid Sequence
Ac-(Sox)PGSFRRR-NH2
Substrate Properties
Peptide-Based Substrate
Excitation⁄Emission (nm)
360⁄485
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Product Applications

  • Drug Discovery & Development
  • Proteins, Expression, Isolation & Analysis
KNZ1081
33101b02879cf9102c7fe9aaacc7f399_CmgtBuyProduct