This application calculates the Tm for a primer and gives instructions on how to dilute the primer to a desired concentration. How to use this calculator

Important note
If the PCR primer contains desired mismatches, e.g., for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.

1. Select polymerases

Phusion or Phire polymerase
Taq based polymerase

2. Type or paste your sequence.

3. Enter PCR conditions