This application calculates the Tm for a primer and gives instructions on how to dilute the primer to a desired concentration. How to use this calculator

Important note
If the PCR primer contains desired mismatches, e.g., for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.

1. Select polymerases

2. Type or paste your sequence. In case of multiple calculations please use "Clear" button, otherwise after changes in Primer #1, resubmit Primer #2.

3. Enter PCR conditions

µM

Primer dilution

Amount of primer
µg
Preparing stock solution
µM

µl of H2O or buffer
Dilution
µl

µM

µl of the stock solution

µl of H2O or buffer

Primer dilution

Amount of primer
µg
Preparing stock solution
µM

µl of H2O or
buffer
Dilution
µl

µM

µl of the
stock solution

µl of H2O or buffer