This application calculates the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases. How to use this calculator

Important note
If the PCR primer contains desired mismatches, e.g., for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.

1. Select your DNA polymerase

Phusion or Phire DNA polymerase
Taq-based DNA polymerase

2. Type or paste your primer sequence

3. Enter primer concentration

µM

mM