Thermo Scientific Molecular Cloning
Molecular cloning kits
Ligase independent cloning (LIC)
LIC ensures high-cloning efficiencies of more than 95% and eliminates the need for ligation and restriction enzyme digestion steps.
Recombinant protein sample preparation
Removal of N-terminal fusion tags from recombinant protein preparations.
Quick and convenient preparation of competent E.coli cells for high transformation efficiencies.
TA and blunt-end cloning
PCR cloning based on positive selection for efficient cloning of PCR products generated with any thermostable DNA polymerase.
Direct one-step cloning of PCR products with 3'-dA overhangs generated by thermostable DNA polymerases, which lack proofreading activity.
Video: How to achieve fast and efficient ligation
How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.
Related molecular cloning products
Wide range of DNA Ladders for accurate DNA sizing and quantification in agarose or polyacrylamide gels.
High purity nucleotides for DNA polymerization reactions.
Reverse transcriptases, primers, nucleotides and and kits for efficient cDNA synthesis.
Phusion High-Fidelity DNA Polymerases, kits and master mixes.
Vector & phage DNA, cloning enzymes, and reagents
pUC plasmid and Lambda DNA for use as molecular cloning vectors.
Enzymes for modification of DNA ends: T4 DNA ligase, alkaline phosphatase, PNK, Klenow, T4 DNA Polymerase.
IPTG and X-gal for blue/white colony selection.
Find videos, webinars, articles, and tools in our Molecular biology resource library
Molecular cloning articles
For Research Use Only. Not for use in diagnostic procedures.