Hot-Start DNA Polymerases & Master Mixes—Thermo Scientific
Hot-start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. We offer a range of hot-start enzymes, including Thermo Scientific™ Phire™ DNA Polymerase, which outperforms Taq-based hot-start polymerases. It is faster, extremely robust, and capable of amplifying long DNA fragments with high yields.
Select the best Thermo Scientific hot-start DNA polymerase for your needs
|Routine performance||Enhanced performance|
|Maxima™ Hot Start Taq DNA Polymerase||Phire Hot Start II DNA Polymerase|
|Amplicon size||Up to 5 kb||Up to 20 kb|
|Activation time||4 min||Immediate|
|Extension time||60 s/kb||10–15 s/kb|
*Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
Additional hot-start DNA polymerases
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