Hot-start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. We offer a range of hot-start enzymes, including Thermo Scientific™ Phire™ DNA Polymerase, which outperforms Taq-based hot-start polymerases. It is faster, extremely robust, and capable of amplifying long DNA fragments with high yields.

Select the best Thermo Scientific hot-start DNA polymerase for your needs

  Routine performance Enhanced performance
  Maxima™ Hot Start Taq DNA Polymerase Phire Hot Start II DNA Polymerase
Enhanced specificity
Amplicon size Up to 5 kb Up to 20 kb
Activation time 4 min Immediate
Extension time 60 s/kb 10–15 s/kb
Stand-alone enzyme
Master mix

*Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Additional hot-start DNA polymerases