The Identifiler® Direct Kit is optimized specifically for 1.2mm discs in a 25µl reaction volume. The kit is designed to overcome the inhibition present when processing unpurified samples in a 25µl PCR reaction volume, and increasing the disc size or reducing the PCR reaction volume can cause the quality of the results to deteriorate.

Applied Biosystems conducted studies showing that sampling from the center of the stain generates more reproducible results. In addition, the Identifiler® Direct kit has been validated for 27 PCR cycles, but Applied Biosystems recommends that laboratories perform their own sensitivity studies to optimize the cycle number for their particular instruments and sample type. Additional recommendations on sampling position and how to optimize cycle number are provided in the User Guide.