The buffer and master mix enhancements in the Identifiler® Plus Kit promote more robust amplification – even in samples that do not contain PCR inhibitors. Purified samples amplified with the Identifiler® Plus Kit can yield peak heights 40-100% higher than the Identifiler® Kit, without any increase in the number of PCR cycles or injection times (Figure 3). In addition, the validation of two different cycle number protocols (28 & 29) enables the laboratory to adapt the amplification to standard input samples and those containing low levels of DNA.

Figure 3. Illustration of the improved sensitivity of the Identifiler® Plus Kit. This figure shows the average peak heights (rfu) obtained for 4 different dilutions of a control DNA sample amplified for 28 cycles using the Identifiler® Kit (green bars) and Identifiler® Plus Kit (orange bars). On average, the Identifiler® Plus Kit peak heights were approximately 1.7 times higher than Identifiler® Kit peak heights.