As part of the validation process, Applied Biosystems tested the performance of the PrepFiler™ kit against a standard organic Phenol/Chloroform process and 3 other commonly used commercially available chemistries on a variety of simulated forensic casework samples (prepared from 2 ul blood, 50 ul saliva, and 1 ul semen). The extracts were evaluated for DNA concentration, total quantity of DNA isolated, and purity using the Quantifiler® Human DNA Quantification Kit. An example of the results is shown above.

In all cases, the PrepFiler™ kit matched or exceeded the concentration and total yield of DNA produced by other methods. For certain sample types, the PrepFiler™ kit yielded greater than 100% more DNA than the other technologies tested.