Protein Conjugates for neuron staining

This class of neuron stains comprises fluorescent conjugates of cholera toxin subunit B, wheat germ agglutinin (WGA), and isolectin GS-IB4. Introduced via microinjection or iontophoresis, these probes are fixable and photostable retrograde tracers and offer choices for synaptic studies (WGA) and to differentiate between neuronal subtypes (GS-IB4).


Select protein conjugates for neuron staining

Protein Conjugates for Neuron Staining

  • Exogenous staining
  • Fixable and photostable
  • Choice of retrograde tracers
  • WGA crosses synapses

Cholera toxin subunit B binds to glycosphingolipids in axonal membranes and serves as a retrograde tracer. Wheat germ agglutinin (WGA) conjugates bind to sialic acid and N-acetylglucosaminyl residues in neuronal membranes and serve as retrograde tracers that will also cross synapses. Isolectin GS-IB4 is selective for terminal α-D-galactosyl residues and serves to differentiate between neuronal subtypes.

 Protein Conjugates
Human cheek epithelial cells labeled with Alexa Fluor® 350 wheat germ agglutinin and stained with SYTOX® Green nucleic acid stain.

Selection Guides for Protein Conjugates

 
ReadoutDirect fluorescence imaging of cells with punctate signal
CarrierWheat Germ Agglutinin
Standard filter set(s)Cy®5
Fluorophore/conjugateQdot® 655
Ex/Em (nm)/655
Tissue sectionsYes
In vivo neuronsNo
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingIontophoresis
Staining modeExogenous
Best forCell populations
FixableYes
TransportRetrograde
Photobleaching resistance5 stars
Synaptic transferYes
Gap junction transferNo
BibliographyCitations
Quantity200 µL
Cat. No. Q12021MP

Properites

ReadoutDirect fluorescence imaging of cells with uniform signal distribution, binds to sialic acid and N-acetylglucosaminyl residues in neuronal membranes
CarrierWheat Germ Agglutinin
Tissue sectionsYes
In vivo neuronsNo
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingIontophoresis
Staining modeExogenous
Best forCell populations
FixableYes
TransportRetrograde
Photobleaching resistance5 stars
Synaptic transferYes
Gap junction transferNo
BibliographyCitations

 

Selection Guide

 
Standard filter set(s)
Fluorophore/
conjugate
Ex/Em (nm)
Quantity
Cat. No.
Wheat Germ Agglutinin, Alexa Fluor® 350 ConjugateDAPIAlexa Fluor® 350346/4425 mgW11263
Wheat Germ Agglutinin, Alexa Fluor® 488 ConjugateFITCAlexa Fluor® 488495/5195 mgW11261
Wheat Germ Agglutinin, Alexa Fluor® 555 ConjugateTRITCAlexa Fluor® 555555/5655 mgW32464
Wheat Germ Agglutinin, Alexa Fluor® 594 ConjugateTexas Red®Alexa Fluor® 594590/6175 mgW11262
Wheat Germ Agglutinin, Alexa Fluor® 633 ConjugateTexas Red®Alexa Fluor® 633632/6475 mgW21404
Wheat Germ Agglutinin, Alexa Fluor® 647 ConjugateCy®5Alexa Fluor® 647650/6685 mgW32466
Wheat Germ Agglutinin, Alexa Fluor® 680 ConjugateCy®5.5Alexa Fluor® 680679/7025 mgW32465
 
ReadoutBinds to ganglioside GM1 to label lipid rafts; requires secondary detectionDirect fluorescence imaging of cells, selective for ganglioside GM1
CarrierCholera Toxin Subunit B (Recombinant)
Standard filter set(s)NAFITCTRITCTexas Red®Cy®5
Fluorophore/conjugateBiotin-XXHorseradish peroxidaseAlexa Fluor® 488Alexa Fluor® 555Alexa Fluor® 594Alexa Fluor® 647
Ex/Em (nm)NA495/519555/565590/617650/668
Tissue sectionsYes
In vivo neuronsNo
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingIontophoresis, microinjection
Staining modeExogenous
Best forCell populations
FixableYes
TransportRetrograde
Photobleaching resistanceNA5 stars
Synaptic transferNo
Gap junction transferNo
BibliographyCitations
Quantity100 µg100 µg100 µg100 µg100 µg100 µg
Cat. No.C34779C34780C34775C34776C34777C34778
 
ReadoutSelective for terminal α-D-galactosyl residues; requires secondary detectionDirect fluorescence imaging of cells, selective for terminal α-D-galactosyl residues
CarrierIsolectin GS-IB4 from Griffonia simplicifolia
Standard filter set(s)NAFITCRhodamineTexas Red®Cy®5
Fluorophore/conjugateBiotin-XXAlexa Fluor® 488Alexa Fluor® 568Alexa Fluor® 594Alexa Fluor® 647
Ex/Em (nm)NA495/519578/603590/617650/668
Tissue sectionsYes
In vivo neuronsYes
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingMicroinjection, iontophoresis
Staining modeExogenous
Best forCell populations
FixableYes
TransportBidirectional
Photobleaching resistanceNA5 stars
Synaptic transferNo
Gap junction transferNo
BibliographyCitations
Quantity500 µg500 µg500 µg500 µg5 mg
Cat. No. I21414I21411I21412I21413W32466