Membrane Potential & Other Mitochondrial Assays

A distinctive feature of the early stages of apoptosis is the disruption of the mitochondria, including changes in membrane and redox potential. Life Technologies offers a number of fluorescent probes for analyzing aspects of mitochondrial activity in live cells, including calcium flux, superoxide production, transition pore opening, and membrane potential.

Select mitochondrial apoptosis products

Mitochondrial Apoptosis Assays

  • Real-time assays in live cells
  • Choice of physiological measurements
  • Fixable probes for multiplexing with immunodetection

With minimal disruption of cellular function some assays can be monitored in real time while others are fixable for multiplexing or HCS applications.

  Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator
Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator.

Selection Guide for Mitochondrial Apoptosis Assays

 
MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging
Rhod-2 AM, cell permeant
Image-iT® LIVE Mitochondrial Transition Pore Assay Kit
Readout
Targeted to mitochondria, exhibits fluorescent increase with superoxide concentration
Calcium indicator targeted to mitochondia; increases fluorescence with Ca2+ concentration
Mitochondrial signal decreases with opening of transition pore
Indicator
MitoSOX™ Red
Rhod-2 AM
Hoechst 33342
Calcein
MitoTracker® Red CMXRos
Common filter
RFP/TRITC
TRITC
DAPI
FITC
TRITC
Ex/Em (nm)
510/580
552/577
350/461
494/517
579/599
Photostability
Four stars
Three stars
Four stars
Signal-to-noise ratio
Four stars
NA
Four stars
Bibliography
Citations
Citations
Citations
Fluorescence Microscope
Yes
Yes
Yes
HTS
No
No
No
Live cells
Yes
Yes
Yes
Fixable
No
No
No
Detergent resistant
No
No
Yes
Format
10 x 50 µg
20 x 50 µg
1 kit (1,000 slides)
Cat. No.
M36008
R1245MP
I35103
 
Readout
Dye sequestered by active mitochondria in response to membrane potential
Mitochondrial depolarization causes JC-1 molecules to aggregate shifting from green to red emission
Mitotoxicity assay with orange stain for active mitochondria, cell-impermeant green stain for labeling dead cells, and a nuclear stain for localization
Indicator
TMRM
JC-1
JC-1
Hoechst 33342
Image-iT® DEAD Green™ viability stain
MitoHealth stain
Common filter
TRITC
TRITC
TRITC
DAPI
FITC
TRITC
Ex/Em (nm)
552/576
514/529
514/590
350/461
488/515
550/580
Photostability
three stars
three stars
Four stars
Signal-to-noise ratio
NA
three stars
five stars
Bibliography
Citations
Citations
 
Fluorescence microscope
Yes
Yes
Yes
HCS    
Yes
HTS
No
No
No
Live cells
Yes
Yes
Yes
Fixable
No
No
Yes
Detergent resistant
No
No
No
Protocol    
Microscopy
Format
25 mg
5 mg
2 x 96-well plates
Cat. No.
T669
T3168
H10295
 
Readout
Dye sequestered by active mitochondria in response to membrane potential
Indicator
MitoTracker® Orange
Common filter
TRITC
Ex/Em (nm)
550/580
Photostability
Four stars
Signal-to-noise ratio
NA
Multiplexing
Yes
Live cells
Yes
Fixed cells
No
Fixable
Yes
Detergent resistant
No
Protocol
Microscopy
Format
20 x 50 μg
Cat. No.
M7510