A distinctive feature of the early stages of apoptosis is the disruption of the mitochondria, including changes in membrane and redox potential. Life Technologies offers a number of fluorescent probes for analyzing aspects of mitochondrial activity in live cells, including calcium flux, superoxide production, transition pore opening, and membrane potential.

Select mitochondrial apoptosis products

 

Mitochondrial Apoptosis Assays

  • Real-time assays in live cells
  • Choice of physiological measurements
  • Fixable probes for multiplexing with immunodetection

With minimal disruption of cellular function some assays can be monitored in real time while others are fixable for multiplexing or HCS applications.

 Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator
Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator.

Selection Guide for Mitochondrial Apoptosis Assays

 
ReadoutTargeted to mitochondria, exhibits fluorescent increase with superoxide concentrationCalcium indicator targeted to mitochondia; increases fluorescence with Ca2+ concentrationMitochondrial signal decreases with opening of transition pore
IndicatorMitoSOX™ RedRhod-2 AM/Hoechst 33342CalceinMitoTracker® Red CMXRos
Common filterRFP/TRITCTRITCDAPIFITCTRITC
Ex/Em (nm)510/580552/577350/461494/517579/599
PhotostabilityFour starsThree starsFour stars
Signal-to-noise ratioFour starsNAFour stars
Bibliography Citations CitationsCitations
Fluorescence Microscope
YesYesYes
HCS
   
HTS
NoNoNo
Live cellsYesYesYes
FixableNoNoNo
Detergent resistantNoNoYes
Format10 x 50 µg20 x 50 µg1 kit (1,000 slides)
Cat. No.M36008R1245MPI35103
 
ReadoutDye sequestered by active mitochondria in response to membrane potentialMitochondrial depolarization causes JC-1 molecules to aggregate shifting from green to red emissionMitotoxicity assay with orange stain for active mitochondria, cell-impermeant green stain for labeling dead cells, and a nuclear stain for localization
IndicatorTMRMJC-1JC-1Hoechst 33342Image-iT® DEAD Green™ viability stainMitoHealth stain
Common filterTRITCTRITCTRITCDAPIFITCTRITC
Ex/Em (nm)552/576514/529514/590350/461488/515550/580
Photostabilitythree starsthree starsFour stars
Signal-to-noise ratioNAthree starsfive stars
Bibliography CitationsCitations 
Fluorescence microscope
YesYesYes
HCS
  Yes
HTS
NoNoNo
Live cellsYesYesYes
FixableNoNoYes
Detergent resistantNoNoNo
Format25 mg5 mg2 x 96-well plates
Cat. No.T669T3168H10295