Lysosome Incorporation
(Enlarge)
 

Fusion of a lysosome with the isolation membrane to form the autolysosome is the penultimate step of the autophagic pathway. Lysosomal membrane markers serve as tools for tracking fusion with the autophagosome prior to degradation of the autolysosome contents, whereas cell permeable LysoTracker® dyes target acidic organelles and are localized within the lysosomes in nanomolar concentrations. Both membrane proteins and Lysotracker dyes serve as effective tools for tracking lysosome behavior.

Autophagy describes the segregation and delivery of cytoplasmic cargo, including proteins and organelles, for degradation by hydrolytic enzymes through the lysosomal machinery. It is critical to the healthy functioning of cells and the failure of autophagy is a major reason for the accumulation of cell damage and aging.

Select Lysosomal Markers

Locating and Tracking Lysosomes

Lysosomal membrane markers serve as tools for tracking fusion with the autophagosome prior to degradation of the autolysosome contents. We offer Molecular Probes® CellLight® constructs containing red or green fluorescent proteins for locating and tracking lysosomal membranes.

  • Choice of red or green fluorescent proteins
  • Can be multiplexed with other fluorescent dyes or proteins


See selection guide for Lysosomal Membrane Markers

 	Autophagy Research Human osteosarcoma (U2OS) cells expressing CellLight® Lysosomes-GFP
Human osteosarcoma (U2OS) cells expressing CellLight® Lysosomes-GFP.

Locating and Tracking Lysosomal Contents

Cell-permeable Molecular Probes® LysoTracker® dyes target acidic organelles in nanomolar concentrations. They are available in a range of colors including deep red to facilitate multiplexing with other fluorescent markers.

  • Highly selective for acidic organelles
  • Range of fluorescent colors
  • Can be multiplexed with other fluorescent dyes or proteins


See selection guide for Markers for Lysosomal Contents

 Co-localization using LysoTracker® Deep Red
Co-localization using LysoTracker® Deep Red.


Selection Guides for Lysosomal Markers

 
TargetLysosomal membranes
ReadoutLocalization of lysosomes by fluorescent protein imaging
Fluorescent labelGFPRFP
Standard filter setFITCTRITC
Ex/Em (nm)485/520 555/584
Signal-to-noise ratio
Photostability
BibliographyCitations
MultiplexingYes
Live cellsYes
Fixed cellsNo
FixableYes
Format1 kit1 kit
Cat. No.C10507C10504
 
TargetTargets acidic organelles at nanomolar concentrations
ReadoutLocalization of lysosomes by fluorescence imaging
Fluorescent labelLysoTracker® GreenLysoTracker® RedLysoTracker® Deep Red
Standard filter setFITCTRITCCy®5
Ex/Em (nm)504⁄511 577/590 647/668
Signal-to-noise ratio
Photostability
BibliographyCitations
MultiplexingYesYesYes
Live cellsYesYesYes
Fixed cellsNoNoNo
FixableYesYesNo
Format20 x 50 uL20 x 50 uL5 x 50 uL
Cat. No.L7526L7528L12492