Light Cubes

Specialty Light Cubes

 
DAPI
 
RFP
 
CFP-YFP
 
Qdot® 605
 
TagBFP
 
Texas Red®
 
AO
 
Qdot® 625
 
CFP
 
Cy5
 
AOred
 
Qdot® 655
 
GFP
 
Cy7
 
Qdot® 525
 
Qdot® 705
 
YFP  
 
Qdot® 545
 
Qdot® 800
   
 
Qdot® 565
 
Qdot® 525-800
  
 
Qdot® 585 



DAPI (357/44 nm Excitation; 447/60 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
1,8-ANS 372480
4-methylumbelliferone360440
7-amino-4-methylcoumarin (AMC)351430
7-hydroxy-4-methylcoumarin360449
ABQ344445
Acridine362462
Alexa Fluor® 350346442
Alexa Fluor® 405 401421
AMCA        345425
AMCA-X 353442
BFP382448
CellTrace™ Calcein Blue373440
CellTrace™ Calcein Violet 401451
Calcofluor White350440
Cascade Blue® 377420
Dansyl   335525
DAPI345455
DiFMU (pH 9.0)357450
DyLight® 350353432
ER-Tracker™ Blue-White DPX371554
Fast Blue360440
Fluospheres® Blue356412
Hoechst 33258345487
Hoechst 33342347483
LIVE/DEAD® Fixable Blue Dead Cell Stain344442
LysoSensor™ Blue374424
LysoTracker® Blue373422
Marina Blue® 365460
Methoxycoumarin360410
Monochlorobimane(mBCI)380461
NucBlue® Fixed Cell Stain345455
NucBlue® Live Cell Stain347483
Pacific Blue™ 405455
PBFI360420
Phorwite AR360430
Quinine Sulfate349461
sgBFP™ (Super Glow BFP)387450
SITS365460
SpectrumBlue405449
Thioflavin TCN350460
Thiolyte® 378483
True Blue365425
Uvitex SFC365435
Y66H360442



TagBFP (390/18 nm Excitation; 447/60 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor® 405401421
DyeLight® 405 400420
BFP    382448
Cascade Blue®     400420
Pacific Blue™ 410455
SpectrumBlue400450
TagBFP  402457



CFP (445/45 nm Excitation; 510/42 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Auramine O  445500
CellLights® CFP434477
ECFP434477
Evans Blue460515
Lucifer yellow 428544



GFP (470/22 nm Excitation; 510/42 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
5-carboxyfluorescein(5-FAM)492518
Acridine Orange + DNA500526
ActinGreen™ (Alexa Fluor 488® phalloidin)496518
Alexa Fluor® 430432540
Alexa Fluor® 488496518
ATTO 465453507
Auramine460540
Aurophosphine470540
BCECF499540
BODIPY® 492/515490540
BODIPY® 505/515502540
BODIPY® FL502540
BTC464540
Calcein494540
Calcium Green™-1506540
Catskill Green 540482540
CellLights® GFP488507
CellEvent™ Caspase-3/7 Green488507
CellTracker™ Green490540
CellRox® Green485520
CFDA494540
CFP434540
Click-iT® EdU Alexa Fluor® 488496518
Cy2492540
CyQUANT® Direct (CyQUANT® GR)500540
DAF-FM493540
DiO (Vybrant® DiO) 489506
Diversa Green-FP 484507
DyLight®+C110 488493518
Emerald Green490540
Evans Blue460515
FITC (Fluorescein)492520
Fluo-3 506540
Fluo-4494540
Fluoro-Emerald494518
Fluoro-Jade® B 480525
Fluor-X   494520
FluoSpheres® Yellow-Green501513
GFP (EGFP)488507
H2DCFDA (H2-DCF,DCFR)504540
HCS LipidTox™ Green 498506
HPTS (Solvent Green 7)455510
LIVE/DEAD® Fixable Green498526
Lucifer Yellow423543
LysoSensor™ Green448503
LysoTracker® Green504511
Magnesium Green™ 506530
mHoneydew478561
MitoTracker® Green FM490512
NBD Amine450530
NBD-X467538
Neurotrace® 500/525 Green497524
Nitrobenzoxadiazole465510
NucGreen™ Dead Cell Stain504523
Oregon Green® 488498524
Oregon Green® 514512532
pHrodo™ Green509533
Premo™ FUCCI Cell Cycle Sensor (S/G2/M phases)474509
Qdot® 525UV525
Qdot® 545UV543
Quant-iT™ PicoGreen® 502522
Rhodamine 110496520
Rhodamine 123507529
Rhodamine Green™ 497523
Rhodol Green™ 496523
rsGFP (red shifted GFP, S65T)498516
S65C479507
S65L484510
S65T488511
sgGFP™ (Super Glow GFP)474509
Sodium Green™506532
SpectrumGreen498522
Surf Green EX469526
SYBR® Gold495539
SYBR® Green I497521
SYBR® Safe507524
SYTO® 9482500
SYTO® 11506525
SYTO® 13487505
SYTO® 16490520
SYTO® 45452484
SYTO® RNASelect™503527
SYTOX® Green504523
TET™520541
TO-PRO®-1515530
TOTO®-1513531
Vybrant® DyeCycle™ Green505532
wtGFP (wild type GFP, non-UV excitation)475509
YFP512529
YO-PRO®-1491507
YOYO®-1491509



YFP (500/24 nm Excitation; 524/27 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange + DNA500526
EYFP514527



RFP (531/40 nm Excitation; 593/40 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
ActinRed™ 555 (rhodamin pphalloidin)540565
Alexa Fluor® 532532554
Alexa Fluor® 546561572
Alexa Fluor® 555555565
Amplex® Red571583
Amplex® UltraRed568583
BOBO™-3570602
Bodipy® TMR544569
Calcium Orange™ 549575
Carboxy SNARF®-1548587
CellLights® RFP552585
CellMask™ Orange556571
CellRox® Orange545565
CellTracker™ Orange  547575
CellTracker™ Red 585612
Cy®3     552570
Cy®3.5      579597
DiI (CellTracker™ DiI)       551568
DsRed Monomer556586
DsRed2      563582
DsRed-Express        557579
dTomato                    554581
DyLight® 594      562576
Eosin    524545
Ethidium Bromide521602
Ethidium Homodimer-1 (EthD-1)530618
Fluospheres® Orange539560
FocalCheck™ Double Orange540555
HCS LipidTox™ Red   582615
HEX™533558
JOJO™-1529545
LIVE/DEAD® Fixable Red593613
mBanana540553
MitoTracker® Orange CMTMRos(MitoTracker® Orange CM-H2TMRos)551576
mOrange548568
mTangerine568585
Nile Red551636
OFP546568
pHrodo™ Red559586
Phycoerythrin (PE, R-phycoerythrin)567576
POPO™-3532573
PO-PRO™-3539570
Premo™ FUCCI Cell Cycle Sensor (G1 phase)555584
Propidium Iodide (PI)530618
Resorufin570585
RFP552585
Rhod™-2551576
Rhodamine Phalloidin557576
Rhodamine Red560580
ROX™ (6-ROX)568595
SpectrumOrange     559588
Sulforhodamine 101578593
SYTOX® Orange567570
TagRFP555584
TAMRA™565580
TET™521542
Tetramethylrhodamine (TRITC)555580
TurboRFP 553574
Vybrant® DyeCycle™ Orange518562
X-Rhod-1570576



Texas Red® (585/29 nm Excitation; 624/40 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
2-dodecylresorufin582595
5-ROX577603
7-AAD549644
Alexa Fluor® 568578603
Alexa Fluor® 594590618
Alexa Fluor® 610610629
Amplex® Red570583
Amplex® Ultra Red567582
BOBO™-3571606
Bodipy® 630/650-X625641
Bodipy® TR-X588621
Calcium Crimson™589608
CellTrace™ BODIPY® TR methyl esther597625
CellTrace™ calcein red-orange575592
CellTracker™ Red CMTPX584613
Click-iT® Alexa Fluor® 594590618
Cy® 3.5581597
DsRed2563588
DyLight® 594593618
Fluospheres® Crimson microspheres620646
Fluospheres® Red microspheres577608
HCS CellMask™ Red stain   587614
HCS LipidTOX™ Red  582615
HCS NuclearMask™ Red 624644
HcRed  594649
Katushka (Turbo FP635)   588635
LIVE/DEAD® Fixable Red Dead Cell stain595613
LysoTracker® Red573592
Magic Red™ 570630
MitoTracker® Red CMXRos578597
mCherry587615
mKate588635
mPlum590649
mRaspberry598625
Nile Red phospholipid551638
pHrodo™ Red559587
ReAsH (TC-ReAsH™)593608
rhodamine Red-X572591
sulforhodamine 101577593
SYTO® 17620635
SYTO® 59622645
SYTO® 61619649
SYTOX® AADvanced™ dead cell stain546647
Texas Red®592615
X-rhod-1579602
YO-PRO®-3612628
YOYO®-3612631

*See the complete list of CellLights® Reagents



Cy®5 (628/40 nm Excitation; 692/40 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor® 633631650
Alexa Fluor® 635634647
Alexa Fluor® 647 650670
Alexa Fluor® 660 663691
Alexa Fluor® 680679704
APC (Allophycocyanin)   651660
BODIPY® 630/650X 626641
BODIPY® 650/665647665
Carboxynaphthofluorescein 600674
CellMask™ Deep Red plasma membrane stain  658676
CellRox® Deep Red640665
Click-iT® Alexa Fluor® 647650670
Cy®5  648666
Cy®5.5678696
DDAO  648658
DiD (Vybrant® DiD)645670
DRAQ5®650680
DyLight 633638658
DyLight 649654673
FluoSpheres® crimson microspheres621646
FluoSpheres® dark red microspheres657683
FxCycle™ Far Red641657
HCS CellMask™ Deep Red stain649670
HCS LipidTOX™ Deep Red634652
HCS NuclearMask™ Deep Red635687
HCS NuclearMask™ Red622644
LIVE/DEAD® Fixable Far Red650665
Lysotracker® Deep Red647668
MitoTracker® Deep Red FM641661
NucRed™ Dead 647642661
NucRed™ Live 647638686
SYTO® 17619638
SYTO® 59621644
SYTO® 60649681
SYTO® 62650681
SYTOX® Red   640658
TO-PRO®-3 642657
TOTO®-3  642661
Vybrant® DyeCycle™ Ruby638686



Cy®5.5 (655/46 nm Excitation; 794/16 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor® 660663691
Alexa Fluor® 680679702
Alexa Fluor® 700696719
ATTO 635635658
ATTO 647644670
ATTO 655663683
ATTO 680680700
ATTO 700699719
Cy®5.5672690
DY-675675699
DyLight® 680676705
FluoSpheres® Dark Red fluorescent microspheres656683
HiLyte Fluor™ 680688702
IRDye 680 NHS Ester683710
IRDye 700 Phosphoramidite691699
Qdot® 705 Nanocrystals300702
Quasa 705688706
SYTO® 60650681



Cy7 (710/40 nm Excitation; 775/46 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
CellVue® NIR780743776
Cy®7745766
DyLight® 750752778
Indocyanine Green768807
IRDye® 800CW774789
LIVE/DEAD® Fixable near-IR750775
XenoLight™ CF770770797

Note: Near Infrared dyes (e.g., IRDye 800CW) work best with low-magnification, high-NA objectives to maximize detection sensitivity.



CFP/YFP (445/45 nm Excitation; 542/27 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
CFP434477Control YFP Emission

Notes: CFP-YFP FRET control. CFP excitation coupled with a YFP emission filter. For FRET sensitized-emission donor-acceptor bleedthrough measurements. Requires CFP and YFP light cubes for CFP-YFP FRET experiments.



AO (442/46 nm Excitation; 488LP nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange + RNA460650
Acridine Orange + DNA500526
Alexa Fluor® 430434540
AmCyan548489
Auramine O  460550
CTC Formazan450630
Di-8 ANEPPS468635
DiA (FAST DiA™)491613
Lucifer Yellow428544
LysoSensor™ Green (pH 5)442505
PerCP488675

Notes: Acridine Orange, longpass emission. To visualize Acridine Orange in green and orange/red emission wavelengths, we recommend using this light cube with the EVOS® FL color or EVOS® FL Auto cell imaging systems. The AO cube passes simultaneous green, yellow, and red fluorescence, which requires color camera detection. Works well with other fluorophores listed below in either monochrome or color camera EVOS® FL cell imaging systems, provided the sample is labeled with only one dye.



AOred (442/46 nm Excitation; 628/32 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange + RNA460650
Acridine Orange + DNA500526*
CTC Formazan450630
Di-8 ANEPPS468635
DiA491613
Fura Red™  (high Ca2+)436637
Lucifer yellow428544

Notes: Acridine Orange, red emission. Can be used with either EVOS® FL color or EVOS® FL monochrome Cell Imaging Systems. Detects red fluorescence, which can be visualized with either a color or a monochrome camera.

*Poor spectral match. Use the GFP or YFP light cube instead.



Qdot® 525 (445/45 nm Excitation; 525/15 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange (+DNA)500526
Alexa Fluor®  430432540
Aiexa Fluor®  488496518
BCECF (pH 5.2)499540
BOBO™-1570602
Calcein494540
Carboxyfluorescein (5-FAM)492518
CellTracker™ Green CMFDA490540
CellTracker™ Violet BMQC415516
CFP434540
Cy2492540
CyQUANT® Direct500540
DAF-FM493540
DiA456590
DiO484501
F2N12S405530, 585
FITC492520
Fluo-4494540
FM® 1-43510626
GFP488507
HCS CellMask™ Green493516
LIVE/DEAD® Fixable Green498506
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
LysoSensor™ Green448503
LysoSensor™ Yellow/Blue (pH 8.0)329440
LysoTracker® Green504511
MitoTracker® Green FM490512
NBD-X467538
NeuroTrace® 500/525 Nissl stain497524
Oregon Green® 488498524
Pacific Orange™ 440551
POPO™-1434456
PO-PRO™-1435455
Qdot® 525UV525
Rhodamine 110496520
Rhodamine Green497523
SYBR® Gold495539
SYBR® Green I497521
SYTO® 9482500
SYTO® 13487505
SYTO® 16490520
SYTO® 45452484
SYTO® RNASelect™503527
SYTOX® Blue444480
ThiolTracker™ Violet405526
YO-PRO®-1491507
YOYO®-1491509



Qdot® 545 (445/45 nm Excitation; 545/22 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor®  430432540
BCECF (pH 5.2)499540
BOBO™-1570602
Calcein494540
CellTracker™ Green CMFDA490540
CellTracker™ Violet BMQC415516
CFP434540
Cy2492540
CyQUANT® Direct500540
DiA456590
DiO484501
F2N12S405530, 585
FITC492520
FM® 1-43510626
GFP488507
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
LysoSensor™ Green448503
LysoSensor™ Yellow/Blue (pH 8.0)329440
MitoTracker® Green FM490512
NBD-X467538
NeuroTrace® 500/525 Nissl stain497524
Oregon Green® 488498524
Pacific Orange™ 440551
Qdot® 545UV545
Rhodamine 110496520
Rhodamine Green497523
SYBR® Gold495539
SYBR® Green I497521
SYTO® 9482500
SYTO® 13487505
SYTO® 16490520
SYTO® 45452484
SYTO® RNASelect™503527
ThiolTracker™ Violet405526
YO-PRO®-1491507
YOYO®-1491509



Qdot® 565 (445/45 nm Excitation; 565/16 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor®  430432540
BCECF (pH 5.2)499540
CellTracker™ Violet BMQC415516
CFP434540
Cy2492540
Di-8-ANEPPS465635
DiA456590
F2N12S405530, 585
FM® 1-43510626
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
LysoSensor™ Green448503
LysoSensor™ Yellow/Blue (pH 8.0)329440
NBD-X467538
NeuroTrace® 500/525 Nissl stain497524
Pacific Orange™ 440551
Qdot® 565UV565
SYBR® Gold495539
SYBR® Green I497521
SYTO® 9482500
SYTO® 13487505
SYTO® 16490520
SYTO® 45452484
SYTO® RNASelect™503527
ThiolTracker™ Violet405526
YOYO®-1491509



Qdot® 585 (445/45 nm Excitation; 586/20 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor®  430432540
BCECF (pH 5.2)499540
CellTracker™ Violet BMQC415516
Di-8-ANEPPS465635
DiA456590
F2N12S405530, 585
FM® 1-43510626
Fura Red (+Ca2+)436655
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
NBD-X467538
Nile red (triglyceride)552636
Pacific Orange™ 440551
Qdot® 585UV585
SYPRO® Ruby450618
ThiolTracker™ Violet405526



Qdot® 605 (445/45 nm Excitation; 505/15 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Alexa Fluor®  430432540
BCECF (pH 5.2)499540
Di-8-ANEPPS465635
DiA456590
F2N12S405530, 585
FM® 1-43510626
Fura Red (+Ca2+)436655
Fura Red (-Ca2+)473670
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
NBD-X467538
Nile red (triglyceride)552636
Pacific Orange™ 440551
Qdot® 605UV605
RH 795530712
SYPRO® Ruby450618



Qdot® 625 (445/45 nm Excitation; 625/15 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Di-8-ANEPPS465635
DiA456590
F2N12S405530, 585
FM® 1-43510626
Fura Red (+Ca2+)436655
Fura Red (-Ca2+)473670
Lucifer Yellow423543
Qdot® 625UV625
RH 795530712
SYPRO® Ruby450618



Qdot® 655 (445/45 nm Excitation; 655/15 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange (+RNA)460650
Di-8-ANEPPS465635
DiA456590
FM® 1-43510626
FM® 4-64558734
Fura Red (+Ca2+)436655
Fura Red (-Ca2+)473670
Qdot® 655UV655
RH 795530712
SYPRO® Ruby450618



Qdot® 705 (445/45 nm Excitation; 705/40 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Di-8-ANEPPS465635
FM® 4-64558734
Fura Red (+Ca2+)436655
Fura Red (-Ca2+)473670
Qdot® 705UV705
RH 795530712
SYPRO® Ruby450618



Qdot® 800 (445/45 nm Excitation; 785/62 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
FM® 4-64558734
Qdot® 800UV800



Qdot® 525-800 (445/45 nm Excitation; 525-800 nm Emission)

  Dye Abs Peak Em Peak Spectral Match
Acridine Orange (+DNA)500526
Acridine Orange (+RNA)460650
Alexa Fluor®  430432540
Aiexa Fluor®  488496518
BCECF (pH 5.2)499540
BOBO™-1570602
Calcein494540
Carboxyfluorescein (5-FAM)492518
CellTracker™ Green CMFDA490540
CellTracker™ Violet BMQC415516
CFP434540
Cy2492540
CyQUANT® Direct500540
DAF-FM493540
Di-8-ANEPPS465635
DiA456590
DiO484501
F2N12S405530, 585
FITC492520
Fluo-4494540
FM® 1-43510626
FM® 4-64558734
Fura Red (+Ca2+)436655
Fura Red (-Ca2+)473670
GFP488507
HCS CellMask™ Green493516
LIVE/DEAD® Fixable Green498506
LIVE/DEAD® Fixable Yellow405575
Lucifer Yellow423543
LysoSensor™ Green448503
LysoSensor™ Yellow/Blue (pH 8.0)329440
LysoTracker® Green504511
MitoTracker® Green FM490512
NBD-X467538
NeuroTrace® 500/525 Nissl stain497524
Nile red (triglyceride)552636
Oregon Green® 488498524
Pacific Orange™ 440551
POPO™-1434456
PO-PRO™-1435455
Qdot® 525UV525
Qdot® 545UV545
Qdot® 565UV565
Qdot® 585UV585
Qdot® 605UV605
Qdot® 625UV625
Qdot® 655UV655
Qdot® 705UV705
Qdot® 800UV800
RH 795530712
Rhodamine 110496520
Rhodamine Green497523
SYBR® Gold495539
SYBR® Green I497521
SYPRO® Ruby450618
SYTO® 9482500
SYTO® 13487505
SYTO® 16490520
SYTO® 45452484
SYTO® RNASelect™503527
SYTOX® Blue444480
ThiolTracker™ Violet405526
YO-PRO®-1491507
YOYO®-1491509