Regular instrument calibration and adjustment are required for high-precision imaging of fluorescent probes, particularly in multicolor applications such as confocal laser scanning microscopy that involve multiple exposures, repetitive scans, or three-dimensional sectioning.

We offer a range Molecular Probes® calibration products to help you achieve optimum performance from your imaging system:

Imaging Dye Standards

Fluorescence intensities are influenced by a variety of sampler- and instrument-related parameters. Consequently, comparisons between data acquired on different instruments or with long intervals (several days or more) between measurements must be made with reference to standard fluorescent materials.  

The Molecular Probes® Reference Dye Sampler Kit provides samples of five extensively characterized fluorescence standards with emission spectra covering the entire visible wavelength range.

In addition, we offer a Molecular Probes® NIST-traceable standard solution directly traceable to SRM 1932. This fluorescein standard can be used as a reference material for calibrating instruments or for determining molecules of equivalent soluble fluorophore (MESF) values for experimental solutions.

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Bovine pulmonary artery endothelial cells (BPAEC). FluoCells® prepared slide #2.


FocalCheck™ Calibration Beads

Our Molecular Probes® FocalCheck™ fluorescent microspheres
are specifically designed for examining the alignment, sensitivity,
and stability of confocal laser scanning microscopes. Several different multicolored configurations of fluorescent ring stains and/or contrasting fluorescent stains are available. The sharp ring stains produce a striking visual representation of instrument misalignment or other aberrations, making them ideal reference standards.

  • Matched to laser lines and common filters
  • Confirm optical sectioning thickness
  • Test alignment and spectral separation

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FocalCheck™ Test Slides

FocalCheck™ fluorescence microscope test slides are specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. Each slide contains 10 sample areas coated with proprietary fluorescent microspheres designed specifically for microscopy applications.
  • FocalCheck™ fluorescence microscope test slide #1 is ideal for alignment, intensity, and calibration
  • FocalCheck™ fluorescence microscope test slide #2 is ideal for evaluating spectral imaging systems
  • FocalCheck™ fluorescence microscope test slide #3 is useful for basic evaluation of filter performance

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A double-labeled microsphere from the FocalCheck™ DoubleGreen Fluorescent Microsphere Kit.


InSpeck™ and TetraSpeck™ Microspheres

InSpeck™ Microscope Image Intensity Calibration Kits contain microsphere suspensions for use as fluorescence intensity reference standards.

TetraSpeck™ Fluorescent Microspheres facilitate the chromatic registration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment.

TetraSpeck™ beads are available in five diameters, spanning the range from subresolution to nearly cell-size particles. They are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks.

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Four exposures of three TetraSpeck™ beads.


FluoCells® Prepared Slides

FluoCells® prepared microscope slides contain multilabeled cell or cryosection preparations for observation by epifluorescence or confocal microscopy. The multicolor staining in these cell preparations results in stunning, publication-quality images. The slides are especially useful for setting up microscopes and camera systems, and for assessing the capabilities of optical filter sets.

  • FluoCells® Prepared Slide #1 (BPAE Cells with MitoTracker® Red CMXRos, Alexa Fluor® 488 Phalloidin, DAPI)
  • FluoCells® Prepared Slide #2 (BPAE Cells with Mouse Anti-α-tubulin, BODIPY® FL Goat Anti-Mouse IgG, Texas Red®-X Phalloidin, DAPI)
  • FluoCells® Prepared Slide #3 (Mouse Kidney Section with Alexa Fluor® 488 WGA, Alexa Fluor® 568 Phalloidin, DAPI)
  • FluoCells® Prepared Slide #4 (Mouse Intestine Section with Alexa Fluor® 350 WGA, Alexa Fluor® 568 Phalloidin, SYTOX® Green dye)
  • FluoCells® Prepared Slide #6 (Muntjac Cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor® 555 Goat Anti-Mouse IgG, Alexa Fluor® 488 Phalloidin, TO-PRO®-3 dye)

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FluoCells® prepared slide #1.

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Sku Name Size Price Qty
F36915 Fluorescein - NIST-Traceable Standard - Nominal concentration 50µM - Special Packaging 5 x 1 mL USD 275.00
F1300 Fluorescein - Reference Standard 1 g USD 115.00
R14782 Reference Dye Sampler Kit - Five 1 mM Solution, 1 mL each 1 kit USD 165.00
Sku Name Size Price Qty
F36909 FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration 1 each USD 306.00
F36913 FocalCheck™ Fluorescence Microscope Test Slide #2, for spectral imaging systems 1 each USD 306.00
F36914 FocalCheck™ Fluorescence Microscope Test Slide #3, 5 colors, high & low intensities 1 each USD 306.00
Sku Name Size Price Qty
M7901 MultiSpeck™ Multispectral Fluorescence Microscopy Standards Kit (in suspension) 1 kit USD 246.00
I7221 InSpeck™ Blue (350/440) Microscope Image Intensity Calibration Kit, 2.5 µm 1 kit USD 176.00
I7225 InSpeck™ Deep Red (633/660) Microscope Image Intensity Calibration Kit, 2.5 µm 1 kit USD 176.00
I7219 InSpeck™ Green (505/515) Microscope Image Intensity Calibration Kit, 2.5 µm 1 kit USD 176.00
I14785 InSpeck™ Green (505/515) Microscope Image Intensity Calibration Kit, 6 µm 1 kit USD 210.00
I7223 InSpeck™ Orange (540/560) Microscope Image Intensity Calibration Kit, 2.5 µm 1 kit USD 176.00
I14786 InSpeck™ Orange (540/560) Microscope Image Intensity Calibration Kit, 6 µm 1 kit USD 176.00
I7224 InSpeck™ Red (580/605) Microscope Image Intensity Calibration Kit, 2.5 µm 1 kit USD 176.00
I14787 InSpeck™ Red (580/605) Microscope Image Intensity Calibration Kit, 6 µm 1 kit USD 176.00
C14837 Constellation™ Microspheres for Imaging, mixture of assorted sizes & colors 3 mL USD 74.00
T7284 TetraSpeck™ Fluorescent Microspheres Sampler Kit 1 kit USD 201.00
T14792 TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide) 1 kit USD 261.00
T7279 TetraSpeck™ Microspheres, 0.1 µm, fluorescent blue/green/orange/dark red 0.5 mL USD 215.00
T7280 TetraSpeck™ Microspheres, 0.2 µm, fluorescent blue/green/orange/dark red 0.5 mL USD 215.00
T7281 TetraSpeck™ Microspheres, 0.5 µm, fluorescent blue/green/orange/dark red 0.5 mL USD 215.00
T7282 TetraSpeck™ Microspheres, 1.0 µm, fluorescent blue/green/orange/dark red 0.5 mL USD 215.00
T7283 TetraSpeck™ Microspheres, 4.0 µm, fluorescent blue/green/orange/dark red 0.5 mL USD 215.00