High-content screening (HCS) or high-content analysis (HCA) describes a set of analytical methods using automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. Thermo Scientific™ High-Content Analysis Products have directly impacted every segment of biological research into cellular and systems biology and drug discovery.

See how researchers like you are using our HCS systems to generate results and publications

Photo of CellInsight™ CX5 HCS Platform
Photo of ArrayScan™ HCA Readers

High-content analysis reagents

BPAE cells imaged using the CellInsight™ CX5

BPAE cells imaged using the CellInsight™ CX5 platform and stained with DAPI (blue), Alexa Fluor® 568 phalloidin (red), and Goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate (green).

HeLa cells imaged using the CellInsight™ CX5

HeLa cells imaged using the CellInsight™ CX5 platform and stained with Hoechst® 33342 (blue) and Alexa Fluor® 680 phalloidin (yellow).

HT1080 cell image acquired using an ArrayScan™ reader

HT1080 cell image acquired using an ArrayScan™ system and stained with DAPI (blue), Alexa Fluor® 555 phalloidin (red), and anti-tubulin and Alexa Fluor® 647 goat anti–mouse IgG (green).

HCA reagent selection guides

Drawing on decades of experience in fluorescence imaging, Molecular Probes® HCS products are developed using Thermo Scientific™ High-Content Platforms with special considerations for the high-throughput workflow and automated imaging:

  • Cell and nuclear masks for automated demarcation
  • Robust functional probes for cell health interrogation
  • Flexible assay workflow for automated processing
  • Broad fluorophore choice for easy multiplexing
  • Validated on multiple imaging platforms

Select from the reagents optimized for use with your HCA platform:

Other cell structure and segmentation tools

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Molecular Probes® cell structure labels are widely used in microscope imaging and HCS.

See reagents for cell structure

Other cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.

Browse cell health and cytotoxicity assays by application: