Antibodies for Flow Cytometry
We offer an extensive catalog of highly specific RUO, ASR, and IVD primary antibodies to a wide range of relevant targets. These antibodies are validated for flow cytometry and include CD markers, antibodies for oncology and immunology research, stem cell studies, cell signaling, cytokines and chemokines, as well as for key targets involved in cellular processes.
Antibodies for flow cytometry
Multiple dye and fluorescent protein conjugates available
We offer 24 different fluorescent dyes (including the Alexa Fluor® family of dyes, and Pacific Blue™ and Pacific Orange™ dyes), proteins (PE, PerCP, and APC), and Qdot® probes conjugated to a wide range of antibodies to further expand your multiparametric flow cytometry capabilities. This range of dyes is compatible with all flow cytometers.
Color range allows easier multiplexing
The availability of more color options allows researchers to be more efficient in their experiments by obtaining more information with less sample, in less time. Through multiplexing, experimental error is diminished by minimizing the number of samples used to obtain results. Our wide selection of fluorophores allows researchers to select panels with minimal spectral overlap (Figure 1).
Figure 1. Eight-color immunostain combination with low compensation values. Human peripheral blood leukocytes (PBLs) were stained with Qdot® 605–anti-CD4, Qdot® 655–anti-CD3, Qdot® 705–anti-CD45, FITC–anti-CD2, R-PE–anti-CD16+CD56, R-PE–Cy®7-anti-CD19, APC–anti-CD14, APC–Alexa Fluor® 750–anti-CD8. Samples were run on an LSR II flow cytometer. Plots are gated on lymphocytes by side scatter/CD45. Axes are labeled with the filters used; plots are labeled with compensation values.
Researchers can increase the number of detectable parameters and have greater flexibility in their experimental designs by combining Qdot® probes with existing organic dyes. The narrow emission spectra of Qdot® probes allow for minimal compensation when using a single laser excitation source, and the signal from Qdot® probes does not degrade over time like tandem conjugates.