Cell Cycle Analysis With the Attune® Flow Cytometer
Accurate cell cycle phase assignments rely on precise measurements of fluorescence intensity differences between multiple cell populations. The Attune® Acoustic Focusing Flow Cytometer is designed to deliver this precision regardless of the sample throughput rate. You no longer need to sacrifice throughput for sensitivity.
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Precision, Regardless of the Sample Throughput Rate
The figures below show the low-variability data obtained with the Attune® Acoustic Focusing Cytometer, even at high sample rates.
|Figure 3. Minimal data variation at high sample rates with the Attune® Acoustic Focusing Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase, and analyzed at a concentration of 1 x 104 cells/mL on a high-end instrument that uses hydrodynamic focusing, and on the Attune® Acoustic Focusing Cytometer at different sample rates. The left peak in all graphcs reflects cells in G0/G1 phase, while the right peak reflects cells in G2/M phase. Note that as sample rates increase on the instrument that uses hydrodynamic focusing, the width of the G0/G1 and G2/M peaks increase, whereas for the Attune® cytometer the peaks are relatively stable, even at the highest sample rate of 1,000 µL/min. These results are quantitated in Table 1.|
Figure 4. Percent CV of G0/G1 at different flow rates on the Attune® cytometer and a competitor (C) at different smaple rates. Note the minimal change in variability (%CV) for the Attune® Acoustic Focusing Cytometer, even at a high sample rate.
|Sample Rate||%G0/G1||%S*||%G2/M||G0/G1 Ratio||%CV|
More Cell Types
In another cell cycle study, five cell types (listed below) were analyzed with the Attune® cytometer and with a high-end hydrodynamic focusing cytometer (ModFit analysis was performed in each case). The sample throughput rate of 25 µL/min was used for the analysis.
Additional cell types analyzed with the Attune® Flow Cytometer:
- Ramos B CellsKG-1
- KG-1 a Myeloblast Cells
- HL60 Promyeloblast Cells
- Myeloma Cells
- ST486 B Cells