When analyzing leukocytes by flow cytometry, efficient cell lysis of erythrocytes from whole blood samples must performed prior to analysis. Life Technologies offers both Cal-Lyse™ premixed lysis solution for erythrocyte lysing and TransFix® Cellular Antigen Stabilizing Reagent for the stabilizing of leukocytes and the preservation of antigenic sites for accurate analysis by flow cytometry and other applications.

Cal-Lyse™ and High-Yield Lysis Solutions

Cal-Lyse™ premixed lysis solution is specifically formulated to lyse erythrocytes following monoclonal antibody staining of whole blood or bone marrow. Treatment with this reagent leads to both the lysis of red blood cells and the fixation of white cells. Treatment does not affect fluorophore-labeled antibodies bound to leukocytes, and leaves morphological scatter characteristics of leukocytes intact.

High-Yield Lyse is a premixed, fixative-free erythrocyte-lysis solution that can be used to help eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with fluorophore-conjugated monoclonal antibodies; there is no need for a wash step.

Cal-Lyse™ Reagent Selection Guide

Product  Class  Quantity  Cat. No.
Cal-Lyse™ RBC Lysis Solution
IVD*
25 mL
GAS010
Cal-Lyse™ RBC Lysis Solution
IVD*
100 mL
GAS010S100
High-Yield Lyse
GPR*
 500 mL HYL250
*IVD = in vitro diagnostics; GPR = general purpose reagent

TransFix® Cellular Antigen Stabilizing Reagent

TransFix® Cellular Antigen Stabilizing Reagent is a whole blood preservative used for the stabilization of leukocytes and the preservation of antigenic sites for future cellular analysis. Now it's simple to batch-process multiple samples for optimal laboratory workflow. Additionally, blood that has been stabilized with TransFix® reagent can be collected and transported to other sites for analysis.

TransFix® reagent is:

  • Easy to use—just add to whole blood, mix, and store for up to 10 days at 4°C
  • Cost-effective—batch samples to optimize laboratory workflow while maintaining sample integrity
  • Proven—multiple publications demonstrate its effectiveness at the preservation of a broad range of cellular antigens
  • Convenient—compatible with EDTA (K3), ACD, and heparin anticoagulants
  • Flexible—preserves a wide range of samples, including those from human, mouse, rat, sheep, pig, and more

TransFix™ Reagent Selection Guide

Product  Class  Quantity  Cat. No.
TransFix® Cellular Antigen Stabilizing Reagent
GPR*
2 mL
FIX2
TransFix® Cellular Antigen Stabilizing Reagent
GPR*
20 mL
FIX20
TransFix® Cellular Antigen Stabilizing Reagent
GPR*
100 mL FIX100
*GPR = general purpose reagent