Alexa Fluor® 350 dyeAlexa Fluor® 350 dye is a blue-fluorescent dye with moderate photostability and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

Blue-fluorescent probes provide high-contrast discrimination for quantitative imaging applications without compromising performance in the popular red and blue channels. Alexa Fluor® 350 protein conjugates are optimally excited at ~346 nm and produce bright, blue fluorescence at a wavelength slightly shorter than that of AMCA or AMCA conjugates, reducing Alexa Fluor® 350 dye’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.

Life Technologies offers Alexa Fluor® 350 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor® 350 dye dashboard


Initial brightness

 
 
 
 
 
   
    The brightest dye available in the blue channel is best used for high-abundance targets like cytoskeleton markers. Commonly used with Alexa Fluor® 488, 594, and 647 dyes for multiplexing.

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 
 

  350 DAPI 346 442  
 
Laser line

Common filter set

Excitation max

Emission max
 

Alexa Fluor® 350

Fixed and permeabilized muntjac skin fibroblasts were stained with Alexa Fluor® 350 phalloidin, a Mouse anti–ATP Synthase Subunit IF1 Monoclonal Antibody, an anti–α-tubulin antibody, and an anti–cdc6 peptide antibody.

The Mouse anti–ATP Synthase Subunit IF1 Monoclonal Antibody was prelabeled with the Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit (green), the anti–a-tubulin antibody was prelabeled with the Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit (red), and the anti–cdc6 peptide antibody was prelabeled with the Zenon® Alexa Fluor® 647 Mouse IgG1 Labeling Kit (pink). The image was deconvolved using Huygens software (Scientific Volume Imaging).

Alexa Fluor® 350 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol Aldehyde-, ketone, carboxylate or phosphate

Alexa Fluor® dye family

Illuminating research for over 15 years

Alexa Fluor® dyesAlexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.

Resources

  

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with Alexa Fluor® 350 dye and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

   

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.