Green Fluorescent Protein
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

Life Technologies offers a series of GFP CellLight® fusion constructs of signal peptides or cell structure proteins with emGFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments.

To complement these tools, we also offer anti-GFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. All of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and CellLight® fusion proteins.

Green Fluorescent Protein (GFP) dashboard


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Green Fluorescent Protein is a useful expression label for flow cytometry or imaging applications. Weak signals can be retrieved or amplified using an anti-GFP antibody. 

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488 FITC 488 510  
 
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Common filter set

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Definition of terms, and how these values were derived

GFP products

CellLight® reagents

CellLight® reagents are ready-to-use fluorescent protein constructs targeted to specific subcellular structures. These reagents provide a simple and effective method for introducing targeted intracellular labels within living cells. Simply add the reagent to your cells, incubate overnight, and you’re ready to image your cells.

 

Anti-GFP antibodies

Anti-GFP antibodies provide a convenient method for visualizing GFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. Our anti-GFP antibodies are easily incorporated into standard immunostaining protocols for cell and tissue analysis.

Green Fluorescent Protein

NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody. Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI  stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.

BacMam technology

  • Efficient transduction of cell lines, including primary cells and stem cells
  • Safe transduction and lack of observable cytopathic effects
  • Easy modulation of gene expression
  • Expression of multi-component complexes at the right stoichiometry

The BacMam technology is based on double-stranded DNA insect viruses (baculovirus) as vehicles to efficiently deliver and express genes in mammalian cells, including those encoding fluorescent proteins. such as Green Fluorescent Protein. Baculoviruses are well-tolerated and nonreplicating in mammalian cells, so they have an excellent safety profile for routine applications.

Learn more about:

 

Bacmam technology

Live-cell imaging of HeLa cells using CellLight™ mitochondria and talin probes, transduced using BacMam 2.0 technology.

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.