TrypLE

TrypLE ™ reagents are highly purified, recombinant cell-dissociation enzymes that replace porcine trypsin.These reagents are ideal for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin without protocol changes.

TrypLE™ reagents are:

  • Gentle on cells—protect your cell’s surface proteins
  • Stable at room temperature—no need to freeze means they’re ready when you need them
  • Animal origin–free—important if you’re looking for a product without animal-derived components

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TrypLE™ Express—uses significant economies of scale to make TrypLE easily accessible to all researchers

TrypLE™ Select (1X)—manufactured on dedicated animal origin-free equipment

TrypLE™ Select (10X)—for fast dissociation of strongly adherent cells and manufactured on dedicated animal origin-free equipment

Figure 1. Comparison of cell dissociation reagents. Jurkat cells were used to demonstrate the effects of cell dissociation reagents on detection of the cell surface epitope CD2. (A) Time course of treatment with 1X TrypLE ™ Express reagent. There is no reduction in the CD2 fl uorescence signal. (B) Time course of treatment with 0.05% Trypsin-EDTA. There is a time-dependent reduction in CD2 detection.

Gentle and convenient
TrypLE™ reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE™ reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol.

Stable at room temperature
TrypLE™ reagent is room-temperature stable and ready to use when you need it. TrypLE™ cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

TrypLE™ Express reagent

TrypLE™ Express reagent proves that superior performance can be affordable. Significant economies of scale make it even easier to replace trypsin with TrypLE™ Express reagent. For fast, effective cell dissociation that doesn’t harm your cells, use TrypLE™ Express reagent.

TrypLE™ Select (1X) reagent

TrypLE™ Select (1X) reagent is manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York.

Cell release times with TrypLE™ Select (1X) reagent

TrypLE™ Select
Cell line Media Mean time required
for cell release (min)
Mean viability (%)
293F DMEM + 5% FBS 2 97
VERO VP-SFM 5 100
VERO EMEM + 5% FBS 8 100
VERO OptiPRO™ SFM 7 98
CHO-K1 CHO III A 7 95

TrypLE™ Select (10X) reagent

TrypLE™ Select (10X) reagent can be used at full strength to quickly and gently dissociate strongly adherent cell types. TrypLE™ Select (10X) reagent is also manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York. 

Strongly adherent cell release times

TrypLE™ Select 10x TrypLE™ Select
Strongly adherent
cell lines
Media Mean time required
for cell release (min)
Mean
viability (%)
Mean time required
for cell release (min)
Mean
viability (%)
PK-15 EMEM + 10% FBS 11.7 ± 2.0 99.4 ± 0.3 27 98
PK-15 OptiPRO™ SFM N/A N/A 11 99
MDCK OptiPRO™ SFM 15.3 ± 0.8 98.7 ± 0.5 28 98
MDBK DMEM + 5% FBS 8.0 ± 1.3 98.8 ± 0.5 15 100
A549 DMEM + 10% FBS 5.2 ± 0.3 99.1 ± 0.5 9 98
MSC DMEM + 10% MSC FBS 7.8 ± 1.2 98.7 ± 1.3 N/A N/A
MSC StemPro® MSC SFM 8.7 ± 1.5 97.5 ± 0.1 N/A N/A