Simplify downstream protein purification with serum-free CHO Medium

  • Get optimal expression of recombinant proteins
  • Select from chemically defined, protein-free, and serum-free formulations (Table 1)
  • Choose media for suspension or adherent cells

Media Selection Guide

Product

Optimized
for

Applications

Serum free

Animal origin free

Protein free

Drug Master File available

CD CHO  Medium Suspension CHO cells (including CHO-S cells) Growth and production of recombinant proteins in suspension culture

CD CHO Medium AGT™ Dry granular format
of CD CHO Medium
Suspension CHO cells (including CHO-S cells) Growth and production of recombinant
proteins in suspension culture

CD OptiCHO™ Medium Suspension CHO cells (including CHO-S cells) Growth and production of recombinant
proteins in suspension culture

 
CD CHO-A Medium Adherent CHO cells Growth and production of recombinant
proteins in adherent culture

 

CHO-S-SFM II
Suspension CHO cells(including CHO-S cells) Growth and production of recombinant
proteins in suspension culture

 

 

CHO III-A-PFM Adherent CHO cells Growth and production of recombinant
proteins in adherent culture

 


Note: Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. More than one medium formulation (if available) should be evaluated to determine the best option.


Definitions from Table 1

Serum-Free Media (SFM)
GIBCO® serum-free media do not require supplementation with serum, but may contain discrete proteins or bulk protein fractions.

Protein-Free Media (PFM)
GIBCO® protein-free media contain no proteins, but may contain plant or yeast hydrolysates. Many are animal origin free.

Chemically Defined Media (CDM)
GIBCO® chemically defined media contain no proteins, hydrolysates,
or components of unknown composition. These media are
animal origin free and all components have a known chemical structure.

  • Eliminate variability with a completely defined system
  • Improve reproducibility with consistent performance
  • Decrease possibility of contamination by adventitious agents
  • Save time with simplified purification and downstream processing