Simplify downstream protein purification with serum-free CHO Medium

  • Get optimal expression of recombinant proteins
  • Select from chemically defined, protein-free, and serum-free formulations (Table 1)
  • Choose media for suspension or adherent cells

Ordering Information

Sku Name Size Price Qty
10743011 CD CHO Medium (1X) 500 mL USD 46.00
10743029 1X CD CHO Medium, liquid 1,000 mL USD 84.00
12490017 CD CHO AGT™ Medium 1 L USD 63.00
12490025 CD CHO AGT™ Medium 10 L USD 562.00
12681011 1X CD OptiCHO™ Medium, liquid 1,000 mL USD 76.00
12681029 CD OptiCHO™ Medium (1X) 6 x 1,000 mL USD 434.00
12052098 CHO-S-SFM II, with hypoxanthine and thymidine 1,000 mL USD 87.00
31033020 CHO-S-SFM II (without hypoxanthine or thymidine) 500 mL USD 67.00
35050061 GlutaMAX™ Supplement 100 mL USD 30.40
25030081 200 mM L-Glutamine 100 mL USD 23.40
21051024 L-Glutamine 100 g USD 75.00
12563011 TrypLE™ Select Enzyme (1X), no phenol red 100 mL USD 27.00
12563029 TrypLE™ Select Enzyme (1X), no phenol red 500 mL USD 94.25
25300054 0.05% Trypsin-EDTA (1X), phenol red 100 mL USD 11.50
25200056 0.25% Trypsin-EDTA (1X), phenol red 100 mL USD 11.50
17075029 Soybean Trypsin Inhibitor, powder 1 g USD 123.00
15230196 Distilled Water 20 x 100 mL USD 135.00
15230162 Distilled Water 500 mL USD 12.75
15230204 Distilled Water 10 x 500 mL USD 111.00

Media Selection Guide

Product

Optimized
for

Applications

Serum free

Animal origin free

Protein free

Drug Master File available

CD CHO  MediumSuspension CHO cells (including CHO-S cells)Growth and production of recombinant proteins in suspension culture

CD CHO Medium AGT™ Dry granular format
of CD CHO Medium
Suspension CHO cells (including CHO-S cells)Growth and production of recombinant
proteins in suspension culture

CD OptiCHO™ MediumSuspension CHO cells (including CHO-S cells)Growth and production of recombinant
proteins in suspension culture

 
CD CHO-A MediumAdherent CHO cellsGrowth and production of recombinant
proteins in adherent culture

 

CHO-S-SFM II
Suspension CHO cells(including CHO-S cells)Growth and production of recombinant
proteins in suspension culture

 

 

CHO III-A-PFMAdherent CHO cellsGrowth and production of recombinant
proteins in adherent culture

 

Note: Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. More than one medium formulation (if available) should be evaluated to determine the best option.

Definitions from Table 1

Serum-Free Media (SFM)
GIBCO® serum-free media do not require supplementation with serum, but may contain discrete proteins or bulk protein fractions.

Protein-Free Media (PFM)
GIBCO® protein-free media contain no proteins, but may contain plant or yeast hydrolysates. Many are animal origin free.

Chemically Defined Media (CDM)
GIBCO® chemically defined media contain no proteins, hydrolysates,
or components of unknown composition. These media are
animal origin free and all components have a known chemical structure.

  • Eliminate variability with a completely defined system
  • Improve reproducibility with consistent performance
  • Decrease possibility of contamination by adventitious agents
  • Save time with simplified purification and downstream processing