Improve suspension culture expansion, transfection, and protein production in DG44 cells

Several new kits for streamlining protein generation in CHO DG44 (dhfr¯) cells are now available. The cells, media, and transfection reagents are designed for suspension culture, reducing the time needed to get to expressed protein. You'll easily create high density CHO suspension cultures, efficiently transfect in suspension, and express protein; all in chemically defined, animal component-free media systems.

Inherent selection with dhfr¯cells

DG44 cells, dihydrofolate reductase deficient (dhfr¯) derivates of Chinese Hamster Ovary (CHO) cells, are routinely used to establish cell lines for production of recombinant protein. They were developed by Dr. Chasin at Columbia University using gamma rays to eliminate the entire dhfr locus. In non-mutated cells, dhfr is an essential enzyme for de novo synthesis of glycine, purines, and thymidylate. This allows dhfr to be used as a dominant selectable marker and a gene amplifier for the expression of proteins in dhfr¯ cell lines. The dhfr¯ mutation in DG44 cells is stable and irreversible, which makes it a suitable mammalian cell line for production of recombinant proteins. GIBCO™ offers a complete system for the growth and expansion of DG44 (dhfr¯) cells in suspension, including:

  • DG44 Cells and Media Kit—fully characterized DG44 cells pre-adapted to chemically defined, animal origin component-free media for growth
  • DG44 Transfection Kit—a complete kit optimized for high-efficiency transfection of CD44 cells in suspension
  • Growth Optimization Kits—two kits that allow you to conveniently optimize growth conditions for your specific applications

Improve suspension culture expansion, transfection, and protein production in DG44 cells. Several new kits for streamlining protein generation in CHO DG44 (dhfr¯) cells are now available. The cells, media, and transfection reagents are designed for suspension culture, reducing the time needed to get to expressed protein. You'll easily create high density CHO suspension cultures, efficiently transfect in suspension, and express protein; all in chemically defined, animal component-free media systems.

Optimize Growth with the DG44 Cells and Media Kit


The DG44 Cells and Media Kit is optimized for suspension growth of CHO-DG44 (dhfr¯) cells and expression of recombinant proteins in suspension culture. This kit provides characterized DG44 cells selected for superior cell growth and transfection efficiencies and CD DG44 Medium, an animal origin component-free, chemically defined formulation that contains no proteins, hydrosylates, or components of unknown composition. CD DG44 Medium is supplemented with necessary components for the growth and maintenance of dhfr¯ cell lines, including hypoxanthine and thymidine. GIBCO™ DG44 Cells are pre-adapted to this medium and grow in suspension culture with densities of ~2-3 × 106 cells/ml. DG44 cells transfected with a plasmid carrying dhfr as a marker can be grown in this medium without the need for any selectable drug agents, providing a great advantage for the production of diagnostic or therapeutic recombinant proteins.