AIM V Serum Free Medium
  • First commercially available, fully-defined serum-free media for immunological studies
  • The only FDA 510(k) cleared commercial T-cell medium for human ex vivo tissue and cell culture processing applications
  • Lot-to-lot consistency and produced under cGMP


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Superior Performance, No Serum Required

AIM V® Medium does not require human serum for optimal growth and activation of differentiated lymphoid cells.

  • Minimizes risk of viral immunogenicity associated with human serum
  • Contains no added cytokines and lymphokines
  • All proteins in the formulation have the natural human sequence

AIM V® Versus Human Serum Supplemented Medium

Figure 1 compares relative LAK cell lytic activity of lymphocytes maintained in AIMV® medium with lymphocytes maintained in RPMI-1640 supplemented with serum.

      Figure 1:  Comparison of LAK cell lytic activity: AIM V® Medium versus human serum-supplemented medium. Human peripheral blood lymphocytes (PBLs) were cultured in RPMI-1640 with 1,000 units/mL of IL-2 plus 2% human serum type AB. Human PBLs were also cultured in serum-free AIM V® Medium with 600 units/mL of IL-2. Results are expressed as averages of duplicate flasks assayed in triplicate using Daudi cells as targets.

Applications of AIM V® Medium

AIM V® Medium has been tested for broad range of applications in adoptive immunotherapy and cellular immunology research.  See some of the applications here:

LAK cell induction & functionCytokine & antibody effects on LAK cell induction
Immunotherapy of murine hepatic metastasesLymphocyte receptor studies
TIL cell generation in hollow fiber bioreactorsNK cells & NK-like effector populations
T cell activationIn vitro & in vivo model systems for adoptive immunotherapy
Cytokine regulation of effector cellsHIV research
Cytokine receptor & postreceptor events in lymphoid effector cellsMacrophage activation
Hybridoma growth & monoclonal antibody productionAntibody-dependent cellular cytotoxicity
Effector cells in antitumor immunityLong-term cultivation of functional human macrophages
In vitro studies of lymphokines & antibodiesMechanisms of cell-mediated cytotoxicity

Cells Types Grown Using AIM V® Medium

T cell types

  • CD8+ T lymphocytes
  • Polyclonal T cells
  • Peripheral blood lymphocytes (PBL)
  • CD4 cells for HIV patients
  • Tumor infiltrating lymphocytes (TIL)
  • Retroviral transduced PBL
  • CD3 positive NK T cells
  • Antigen-specific cells (not clonal)
  • γδ T cells
  • NK cells

Other cell types

  • Macrophages
  • Human monocytes
  • HUT78 (human)
  • Dendritic cells
  • HL-60
  • COS cells (monkey)
  • YAC-1 (mouse)
  • Murine lymphoma
  • Myeloid cells
  • Leukemic cells
  • Human fibroblasts
  • VERO
  • Oral epithelial

Ordering Information

Sku Name Size Price Qty
31035025 AIM V® Medium (research grade), AlbuMAX® Supplement 500 mL USD 79.00
12055091 AIM V® Medium, liquid (research grade) 500 mL USD 64.50
12055083 AIM V® Medium, liquid (research grade) 1,000 mL USD 114.00
0870112DK CTS™ AIM V® Medium 1,000 mL USD 134.00
0870112BK CTS™ AIM V® Medium 10 L USD 934.00
A1048501 CTS™ OpTmizer™ T Cell Expansion SFM 1,000 mL USD 136.00