Fast Track to Expression and Analysis

  • Vast selection – over 16,000 Ultimate™ Human ORF Clones and over 2,500 Ultimate™ Mouse ORF Clones
  • Gateway® entry clone format – 1-hour recombinatorial cloning into expression vectors gets you to expression and analysis faster
  • 100% amino acid match guarantee – sequence verification against GenBank®, Ensembl, and Swissprot databases

Ultimate™ ORF clones eliminate the initial cloning and verification steps of your gene discovery research. There is no need to perform tedious RNA isolation, cDNA synthesis, PCR amplification, cloning, sequencing, and validation procedures. You will save literally weeks of time by moving directly to the expression and protein analysis steps of your workflow.

Ordering

All Ultimate™ ORF clones can be readily identified and ordered via an online search using the Ultimate™ ORF Browser. This convenient search engine allows you to find your gene of interest using keywords, clone ID, accession number, or sequence information. The clone ID hyperlink allows you to retrieve the Ultimate™ ORF Card where continuously updated information on the clone can be found. For high-volumes orders, please contact our Customer Service Representatives for support.

Expression-ready clones

All Ultimate™ ORFs are provided in the Gateway® entry vector pENTR™221 and they have the amber stop codon that makes them compatible with the Tag-On-DemandTM technology. You can rapidly and efficiently recombine the ORF of interest into any expression vector and go straight to gene analysis in your system of choice. Learn how Gateway® technology can save you time and effort.

Quality Tested & Guaranteed

The Ultimate™ ORF Clone Collection is updated quarterly with the most current information from the GenBank, Ensembl and Swissprot databases and if any Ultimate ORF Clone have become invalid, they are deactivated from the collection and moved to the ORFanage Collection. The Ultimate™ ORF Clones are guaranteed to match GenBank® sequence information 100% at the amino acid level. To ensure that you receive the highest-quality and most scientifically relevant clones, Ultimate™ ORF clones have been full-insert sequenced. In addition, before shipping, the clone culture must pass a stringent QC test that includes end-sequencing to guarantee the identity of the clone.. You can have peace of mind knowing the clone you ordered is the clone you will receive.

One solution for every budget

Whether you are looking at purchasing one gene or a subset of drugable targets from our collection, we have an option for you. Ultimate™ ORF Clones are available in tube format with price discounts based on volume. Also, for large orders we offer cost-effective formats such as the 96-well plates or the Ultimate™ ORF Clone LITE (click here for more details).
Want a quote for an order with more than 96 ORFs? Contact us here (cloning.technologies@lifetech.com, techsupport@lifetech.com)

What You Receive

Your clone will be shipped as an E.coli  glycerol stock on dry ice.  Please store promptly at -80°C.

Life Technologies™ Corporation provides an evolving clone collection where each gene represented is identified as containing a complete open reading frame, based on a dynamic source of bioinformatic information contained within GenBank. GenBank is a genetic sequence database, which contains an annotated collection of all publicly available DNA sequences. Ultimate™ ORF Clones are typically derived from cDNA clones believed to contain a complete open reading frame, according to the most current GenBank update. Please note, subsequent GenBank updates may show some of these cDNA clones to contain a partial sequence, a non-coding sequence or other artifacts. Quarterly, Life Technologies™ Corporation will update the Ultimate™ ORF Clone Collection, by adding, deleting, or modifying the bioinformatic information to more accurately reflect the most current scientific information associated with each of the clones represented in the collection. It is highly recommended that the purchaser carefully evaluate the ORF clone sequence prior to purchase. It is the purchaser’s responsibility to check whether the sequence associated with any particular clone meets his/her scientific requirement prior to purchase. Life Technologies makes no warranties, express or implied, that the manufacture, use, importation, or sale of the gene contained within any Ultimate™ ORF clone will not infringe any third party intellectual property right.