Chemically competent cells are calcium chloride treated to facilitate attachment of the plasmid DNA  to the competent cell membrane. The competent cell is alternatively heated in a water bath, this opens the pores of the cell membrane allowing entry of the plasmid. Chemically competent cells are the best solution for general cloning and subcloning application.

Flexible Packaging Formats

Each project has its own unique requirements, many of Invitrogen’s competent cells are offered in a variety of packaging formats. 

Options available for chemically competent cells:

  • One Shot® – Transformation and recovery in the same tube.
  • MultiShot™ StripWell™  - 12 strips of 8 tubes in a 96 well format; do as many or as few reactions as needed
  • MultiShot™ – 96-well microarray plates fit automated format
  • Standard – Economical option

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High Efficiency Chemically Competent Cells (> 109 cfu⁄µg)

Strain Contains
F' Episome
T1 Resistance
Available
Genotype  
DH10B™     Genotypes Order
DH5α™   Yes Genotypes Order
Mach1™   Yes Genotypes Order
OmniMAX™2 Yes Yes Genotypes Order
Stbl2™     Genotypes Order
TOP10™     Genotypes Order
TOP10F'™ Yes   Genotypes Order
ccb Survival™ 2   Yes Genotypes Order

Medium Efficiency Chemically Competent Cells (108 - 109 cfu⁄µg)

Strain Contains
F' Episome
T1 Resistance
Available
Genotype  
DH10Bac™  GenotypesOrder
DH5α™Yes GenotypesOrder
INVαF'™Yes GenotypesOrder
MC1061/P3   Order
Stbl3™  GenotypesOrder
TOP10™  GenotypesOrder
TOP10/P3   Order

Subcloning Efficiency Chemically Competent Cells (106 - 107 cfu⁄µg)

Strain Contains
F' Episome
T1 Resistance
Available
Genotype  
DH5α™  GenotypesOrder
INV110Yes GenotypesOrder