Invitrogen offers you a comprehensive collection of electrocompetent cells for every cloning project.  Select from our wide range of transformation efficiencies, genotypes for every application, packaging formats for every workflow, and more.  Each of these factors will directly impact the time and effort required for your project, as well as its success.

Electroporation Competent Cells available

Cell Strain

  Description   Application   Transformation Efficiency  
MegaX DH10B™ T1R Electrocomp™ Cells

 MegaX DH10B™ T1R Electrocomp™ cells are the highest-efficiency electrocompetent cells.
See additional details below.
 Ideal for highly demanding cloning and library construction applications >3 x 1010 cfu/μgOrder Now
E-Shot™
DH10B™ T1R  Electrocomp Cells


 Predispensed into convenient, single-use 0.1 cm universal-fit cuvettes, ready for electroporation.  This format of the DH10B™ T1R  strain eliminates preparation and aliquotting steps, saving time. For use in general cloning procedures. >1 × 1010 cfu/μgOrder Now
ElectroMAX™ DH10B™ T1R Cells

 High efficiency cells derived from the widely used DH10B strain tonA genotype for T1 and T5 phage resistance.  Also available in bulk format. Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. >1 × 1010 cfu/μgOrder Now
ElectroMAX™DH10B™ Cells


 Derived from the DH10B™ strain in a high efficiency electrocompetent format. Also available in bulk format. Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. >1 × 1010 cfu/μgOrder Now
ElectroMAX™
DH5α-E™ Cells
 Derived from the DH5α™ stain and are suitable for transformation by electroporation. Also available in bulk format. Procedures requiring high transformation efficiencies, such as generation of cDNA libraries or with limited input DNA. >1 × 1010 cfu/μgOrder Now
One Shot® TOP10 Electropcomp™ Cells Genetically similar to the reliable DH10B strain and offers the added convenience of single-use One Shot® format. Used for general cloning applications.  The TOP10 strain is found in many TOPO® cloning and expression kits >1 × 1010 cfu/μgOrder Now
TOP10 Electropcomp™ Cells Genetically similar to the reliable DH10B strain. Used for general cloning applications.  The TOP10 strain is found in many TOPO® cloning and expression kits >1 × 1010 cfu/μgOrder Now
TOP10F’ Electropcomp™ Cells Genetically similar to the reliable DH10B strain with the F’ genotype.  Used for general cloning applications.  >1 × 1010 cfu/μgOrder Now

Highest-efficiency electrocompetent cells

MegaX DH10B™ T1R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available (Figure 1), with a guaranteed three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC control DNA). They are ideal for highly demanding cloning and library construction applications. MegaX cells have the same genotype as the widely used DH10B™ T1R strain, including tonA to prevent T1 and T5 lytic phage infection and safeguard your valuable clones and libraries; however, they are manufactured using an improved process that results in a significantly higher transformation efficiency. Available in the bulk Electrocomp™ format.

 

 

Figure 1: MegaX  DH10B™  T1R  Electrocomp™  cells consistently outperform the competition. L: E.cloni®  10G SUPREME cells (Lucigen); G:GC10™  Thunderbolt™ cells  (GeneChoice);  S: ElectroTen-Blue®  electroporation-competent cells (Stratagene).

Order Now