ExpressLink™ T4 DNA Ligase
ExpressLink™ T4 DNA Ligase is a T4 ligase that catalyzes the ligation of blunt or cohesive end DNA fragments in only 5 minutes at room temperature (25°C) and PCR fragments with “A” overhangs in 15 minutes, also at 25°C. T4 ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA strands with 3´ hydroxyl and 5´ phosphate termini in the presence of ATP. ExpressLink™ T4 DNA Ligase formulation is optimized for fast ligase reaction times and more convenient incubation temperature than our other formulations. Single-stranded nucleic acids are not substrates for this enzyme
- Fast, 5 minute ligation of blunt or cohesive end DNA fragments
- Fast Ligation of PCR fragments with “A” overhangs in 15 minutes
- Convenient 25°C reaction temperature eliminates the need for waterbath or temperature block.
- Ideal for cloning DNA into vectors, recircularization of linear DNA, library construction, TA cloning, and linker ligation.
- Exonuclease free.