Frequently asked questions

Instrument and reagent related questions

  1. What kind of equipment is required to work with the BenchPro® 2100 Plasmid Processing Station?
  2. Can the BenchPro® 2100 Plasmid Processing Station run on house vacuum?
  3. How many samples can be run simultaneously?
  4. Is the BenchPro® 2100 Plasmid Purification Card reusable?
  5. Is the waste tray reusable? Can it be autoclaved?
  6. Are the cell liner trays reusable?
  7. How should the card and reagent tray be stored?
  8. If I don’t have sufficient house air, can I use an air compressor? If so, what specifications does an external air compressor have to meet?
  9. What warranty do you offer on the BenchPro® 2100 System?


Protocol related questions

  1. What is the total time required for plasmid purification?
  2. Can I change the elution volume to increase the concentration of my plasmid DNA?
  3. What is the size range of plasmids that the BenchPro® 2100 instrument can purify?
  4. Can I purify plasmids from cell pellets?
  5. How much cell mass by weight can be processed using the BenchPro® 2100 instrument?
  6. What is the quality of plasmids obtained from the BenchPro® 2100 instrument?
  7. How do I de-contaminate the BenchPro® 2100 instrument if necessary (in case of culture spills etc)?
  8. How do I improve my yield on the BenchPro® 2100 instrument?
  9. Can we use TB or rich media with the BenchPro® 2100?
  10. Is it possible to elute my sample with water instead of TE?
  11. What strains of E. coli can plasmid DNA be purified from when using the BenchPro®2100?
  12. Can I use BenchPro®2100 to isolate BAC clone?


Software Related Questions

  1. How can I get software updates for the BenchPro® 2100 Plasmid Processing Station?
  2. How do I update my software on the BenchPro® 2100 Plasmid Processing Station?
  3. Can the BenchPro® 2100 Plasmid Processing Station be connected to a laboratory information management system (LIMS)?
  4. Can BenchPro® 2100 Plasmid Processing Station protocol runs be interrupted, and then subsequently be continued to completion?

Answers

Instrument and reagent related questions 


1.  What kind of equipment is required to work with the BenchPro® 2100 Plasmid Processing Station? 

You will need an in-house air supply outlet (80–100 psi (pounds per square inch), 3 cfm (cubic-feet per minute), constant flow rate), or a compressor unit with the same specifications, that can be connected to the BenchPro® 2100 Plasmid Processing Station.


2.  Can the BenchPro® 2100 Plasmid Processing Station run on house vacuum?

No, the instrument works using positive air pressure, and cannot work on vacuum. It works using house air which is the orange line that forces air out. The pressure has to be 80-100 psi.

3.  How many samples can be run simultaneously? 

You can run one or two samples simultaneously on the BenchPro® 2100 Plasmid Processing Station


4.  Is the BenchPro® 2100 Plasmid Purification Card reusable?


No, the BenchPro® 2100 Plasmid Purification Card is not reusable. During the purification E. coli are captured and lysed within the card, making subsequent reuse impossible.
 

5.   Is the waste tray reusable? Can it be autoclaved?

The waste tray is reusable and is made of material that can be autoclaved. You can also order new waste trays.
 

6.  Are the cell liner trays reusable?


No. The cell liner tray and lid are not reusable. They should be disposed after each procedure. Each BenchPro® 2100 reagent kit comes with a supply of cell liner trays and lids. Cell liner trays are not currently available for standalone purchase.


7.  How do you store the card and the reagent tray?

Always store the BenchPro® 2100 Plasmid Card and BenchPro® 2100 reagent tray in the supplied box to prevent any damage to the card sippers and to the tray foil. Store both at room temperature.


8.   If I don’t have sufficient house air, can I use an air compressor? If so, what specifications does an external air compressor have to meet? 

Yes, you can use an air compressor. Please contact Technical Support for recommended models. The air compressor will need to:

  • Regulate pressure output between 80 and 100 psi  
  • Regulate pressure output flow at >3 cfm, when measured at 80 psi
  • Regulate output flow to < 5°C dewpoint.


9. What warranty do you offer on the BenchPro® 2100 System?

With purchase of a BenchPro® 2100, we offer a 1-year manufacturer’s limited warranty. The BenchPro® 2100 Plasmid Processing Station is covered by our Rapid Exchange Service protection. Should your instrument fail, we will send out a factory-certified refurbished replacement instrument within one business day. Typically, you should receive your replacement within 3 business days of shipment. All you need to do is repackage the problem instrument and return it to us. The service also includes full access to our Applications and Instrument Technical Support teams. The service does not include planned maintenance or any other user-replaceable parts. Computer hardware is not covered by this plan.

Additionally, you have the option of extending your warranty period by 1 or 3 years. This would be in addition to the standard 1-year manufacturer's limited warranty. To learn more about our extended warranties for BenchPro®2100, please visit:


Protocol related questions 


1.  What is the total time required for plasmid purification?

The BenchPro® 2100 instrument needs a manual setup time of less than 5 minutes. After setup, the instrument takes about 90 minutes for the entire purification process. You do not need to be present during the purification process. 
 

2.  Can you change the elution volume to increase the concentration of my plasmid DNA? 

Yes. If you wish to obtain a higher concentration of plasmid DNA, you can do so by reducing the volume of elution buffer in the corresponding compartment of the reagent tray. To reduce the volume of elution buffer, use the pipette tip to pierce the foil, then draw out a corresponding volume of buffer. For specific instructions on how to do this, please contact Technical Service. 


3.  What is the size range of plasmids that the BenchPro® 2100 instrument  can purify?

The instrument is capable of purifying plasmids from 2.7 to 20 Kb
 

4. Can I purify plasmids from cell pellets?


 Yes. Resuspend the cell pellets in 100 mL of LB medium before you load the mixture onto the cell liner tray. 


5. How much cell mass by weight can be processed using the BenchPro® 2100 instrument? 

On an average you can process 0.7 to 0.9 grams of wet pellet biomass  on this instrument. However, this is subject to variability when working  with cells of different size and different strains.  


6. What is the quality of plasmids obtained from the BenchPro® 2100 instrument?

The purified plasmid is ultrapure and suitable for all downstream applications, including those requiring the highest purity, such  as, transfection of mammalian cells, automated and manual DNA  sequencing, PCR amplification, in vitro transcription, bacterial cell  transformation, cloning and labeling.


7. How do I de-contaminate the BenchPro® 2100 instrument if necessary  (in case of culture spills etc)? 

You can clean the surface of the BenchPro® 2100 instrument with a  damp cloth. Do not use harsh reagents or solvents to clean the unit.  Clean the bottom of the instrument drawer with a light spray of 70%  ethanol and then wipe the ethanol with a paper towel. 


8. How do I improve my yield on the BenchPro® 2100 instrument? 

For best results, follow these guidelines:

  1. Use bacterial culture at a cell density of approximately 109 cells/mL, or an optical density of 2.0 to 2.4 at 600nm (OD600). Use bacterial  culture in transition between exponential and stationary phase.
  2. Use a high copy-number plasmid to obtain a good yield of plasmid  DNA. High copy-number plasmids typically yield 2–6 µg DNA/mL LB  culture grown overnight


9. Can we use TB or rich media with the BenchPro®2100?

We do not recommend the use of TB or rich media. A high cell density will clog the membrane and decrease DNA yield from the purification. For best results, set up the purification as indicated in the manual.


10. Is it possible to elute my sample with water instead of TE?

Yes. Water can be used to replace the 1.5 TE buffer for elution. Carefully pierce only the TE-containing chamber and remove all TE. Rinse the chamber twice with purified water and then fill with 1.5ml purified water.


11. What strains of E. coli can plasmid DNA be purified from when using the BenchPro®2100?

We have tested DH10B-T1R, DH5α, JM109, TOP10, and XL1-Blue in-house, and expect that any strains derived from these will also work. Note: endA+ strains have been shown to work, but there may be nuclease activity in the final purified plasmid.


12. Can I use BenchPro®2100 to isolate BAC clone?

No. Purification of BAC clones is not optimal due to their large size. The BenchPro®2100 is capable of purifying plasmids from 2.7 to 20kb.


13. Can I use a frozen cell pellet?

Yes. You can re-suspend the pellet in LB media or sterile water such that the OD at 600nm is 2.0.


Software related questions 


1. How can I get software updates for the BenchPro® 2100 Plasmid Processing Station?

By simply using a USB device, you can download any available software updates.


2. How do I update my software on the BenchPro® 2100 Plasmid Processing Station? 

After downloading the software updates, insert your USB device in the  slot on the back of the instrument, and then follow instructions on the  screen.

3. Can the BenchPro® 2100 Plasmid Processing Station be connected to a laboratory information management system (LIMS)?

No, the current version of the BenchPro® 2100  cannot be connected to any laboratory information management systems. 


4. Can BenchPro® 2100 Plasmid Processing Station protocol runs be interrupted, and then subsequently be continued to completion? 

No, the current version of your software will not allow you to interrupt and subsequently continue your run

 

For complete ordering info or to request an instrument demo for your lab.