mRNA for Northern Blotting
Only 1-5% of the total RNA in the cytoplasm of a typical eukaryotic cell is mature mRNA. This mRNA may occur in high, medium or low abundance. By using mRNA instead of total RNA, cleaner results of greater sensitivity are obtained. The reduced background and increased signal intensity give impressive and unambiguous Northerns.
- Isolate mRNA from total RNA or directly from crude samples in only 15 minutes.
- The isolation protocol can be scaled up or down.
- Easy and efficient magnetic handling minimises losses caused by sample manipulations.
- Stringent hybridisation and washing buffers along with strong RNase inhibition assures isolation of full-length, intact mRNA.
- Dynabeads® Oligo(dT)25 can be regenerated and reused at least four times.
Isolate mRNA with Dynabeads®
Dynabeads® Oligo(dT)25 allows the isolation of mRNA directly from crude samples or from total RNA. The flexible and scalable method fits any sample size, and is particularly useful for Northern analysis starting from small amounts of sample material.
The unique paramagnetic properties of Dynabeads® facilitate easy and efficient handling, whilst also eliminating the requirement for centrifugation, precipitation and the use of hazardous chemicals. Using a solid-phase enables all procedures to be conducted in a single tube and ensures that losses caused by multiple sample manipulations are minimised. This is particularly important when working with small and precious samples or when isolating low abundance transcripts.
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