E-Gel Imager

The E-Gel® Imager is a compact, affordable, easy-to-use imaging system for stained gels and WesternDot® 625 probe–stained western blots. Equipped with a high-resolution digital camera, the E-Gel® system can be used to image gels incorporating fluorescent stains (e.g., ethidium bromide and SYBR® stains) or colorimetric stains (Coomassie™ and silver stains). Additionally, you can image western blots detected with the WesternDot® 625 detection kit reagents, avoiding the need for film processing. Setup is simple, and the system features intuitive software for band analysis.

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High-quality imaging from a variety of gel types

The E-Gel® Imager is designed for the documentation and densitometric analysis of stained nucleic acids and proteins in agarose and polyacrylamide gels, including E-Gel® agarose gels and Novex® NuPAGE® acrylamide gels. In addition, the E-Gel® Imager may be used to image western blots that have been probed with WesternDot® 625 secondary antibodies.

Each E-Gel® Imager includes a camera hood and your choice of interchangeable bases:

  1. Blue Light Base—ideal for DNA stains such as SYBR® Safe stain and SYBR® Green stain, as well as for E-Gel® EX agarose gels
  2. UV Light Base—best for ethidium bromide–stained gels
  3. E-Gel® Adaptor Base—use with the E-Gel® Go!™ System or E-Gel® iBase™/E-Gel® Safe Imager System for real-time monitoring of gel progress

You may use either the Blue Light Base or the UV Light Base to image stained protein gels (including SimplyBlue™ Safe, SilverQuest™ Silver, SYPRO® Ruby, and Coomassie™ blue stains) by using the E-Gel® Imager White-Light Conversion Screen

Filter choice for optimum signal capture

Your choice of three interchangeable filters allows even greater flexibility to capture images from various stains—the orange “universal” filter; a green filter optimal for SYBR® Green and SYBR® Safe stains and for E-Gel® EX agarose gels; and an optional red filter for higher resolution when using Molecular Probes® Qdot® 625 probes.

E-Gel Imager filters

Every E-Gel® Imager camera hood comes with a pre-installed orange (universal) filter. Users may remove the orange filter and replace with either a green or red filter, depending on the stain used.

Molecules

Gel type

Stain

E-Gel® Imager System with:

UV Light Base

Blue Light Base

E-Gel® Adaptor Base

Nucleic acids Agarose (incl. E-Gel® gels) and polyacrylamide gels Ethidium bromide ✓✓✓✓    
SYBR® stains1,2 ✓✓ ✓✓✓✓ ✓✓✓✓
Colorimetric stains3 ✓✓✓✓ ✓✓✓✓  
Proteins Polyacrylamide gels Colorimetric stains3 ✓✓✓✓ ✓✓✓✓  
Fluorescent stains4 ✓✓✓✓    
Western blots Qdot® detection5 ✓✓✓✓    
  1. SYBR® stains (i.e., SYBR® Green, SYBR® Safe, and SYBR® Gold stains) are excited most optimally with blue light. If using UV light to excite SYBR® stains, the E-Gel® Imager UV/SYBR® Filter will also be required.
  2. E-Gel® gels may be visualized on the UV Light Base (ethidium bromide, SYBR® Safe, or SYBR® Gold stained gels), Blue Light Base (SYBR® Safe or SYBR® Gold stained gels), or E-Gel® Adaptor Base (SYBR® Safe or SYBR® Gold stained gels). If you’re using the E-Gel® Adaptor Base, an E-Gel® iBase and an E-Gel® Safe Imager (or combo kit of those items) will also be required.
  3. The E-Gel® Imager White-Light Conversion Screen is required for viewing DNA gels stained with SilverQuest™ Silver Stain for DNA, and protein gels stained with SimplyBlue™ Safe Stain, SilverQuest™ Silver Stain, and Coomassie™ Blue Stain.
  4. Protein gels with stains such as SYPRO® Ruby may be visualized on the E-Gel® Imager System with the UV Light Base.
  5. For higher-resolution imaging results, the E-Gel® Imager Qdot® 625 Filter may be used for imaging western blots probed with Qdot® probes, such as WesternDot® 625 secondary antibodies with VIVID® technology.

E-Gel® Imager Software

The GelQuant™ Express Analysis Software and GelCapture™ Acquisition Software applications integrate seamlessly with the E-Gel® Imager System to provide you with all the applications, functions, and tools for analyzing captured images and data, including densitometric analysis (based on a standard). Both software applications are included with each E-Gel® Imager System.

Excellent detection of target proteins with WesternDot® 625 secondary antibodies

The E-Gel® Imager (with either the orange or red filter) can image WesternDot® secondary antibody–probed blots with sensitivity that is comparable to that observed using enhanced chemiluminescence (ECL)–based western blot detection. WesternDot® secondary antibodies incorporate Qdot® probes enhanced with VIVID® technology, which results in a brighter signal than with the previous-generation Qdot® reagents.

  • Faster time-to-results—eliminate cumbersome HRP and ECL optimization
  • Cost savings—image the blot directly; no more films to process
  • Blot signals stable for weeks to months—obtain multiple exposures without significant reduction in signal

Learn more about WesternDot® 625 secondary antibodies:

Schematic diagram of WesternDot® blot imaging

Western blot detection using (A) WesternDot® secondary antibodies utilizes a more streamlined protocol and results in a more stable signal compared to (B) ECL detection.

western blot dilution series data

A western blot of a dilution series of Jurkat lysate (10 µg to 4 ng) was probed with an anti-GAPDH primary antibody followed by a Qdot® 625 Donkey Anti-Rabbit Secondary Antibody. The blot was imaged on the E-Gel® Imager. When compared to a duplicate western blot detected using ECL followed by imaging on the Fuji FLA-9000 image scanner, the sensitivity was comparable.

Specifications

Dimensions (W x D x H) Hood (20.3 x 28.4 x 36.5 cm)
Base (21.4 x 30.4 x 11.9 cm)
Viewing dimensions (W x D) 12 x 15 cm
Excitation light source UV light–312 nm
Blue light–470 nm
Power 110 or 220  VAC; 50–60 Hz
Camera
      Type CMOS
      Gradation 16-bit (65,536 gray levels)
      Resolution 1280 (H) x 1024 (V); 1.3 megapixels
      Dynamic range 3.8 orders of magnitude
      Exposure time 0.124 sec to 1 min
Optics
      Super bright lens F/1.4 fixed lens
Optional: 16 mm
      Field of view 11 x 14 cm for 16 mm lens
      Emission filter Orange filter (ethidium bromide and SYBR® Safe DNA Gel Stain)
Green filter (Fluorescein, SYBR® Gold, SYBR® Green)
Red filter (Qdot® 625)
Software
      Image capture GelCapture™ Acquisition Software
      1D image analysis GelQuant™ Express Analysis Software
System requirements
      Operating system Windows® XP Professional (English version, SP3, 32-bit), OR
Windows® 7 Professional (English version, 32-bit)
      Processor Intel® Core™2 Duo, 1.8 GHz
      Memory Minimum 1 GB RAM (2 GB recommended)

E-Gel® Imager videos

The E-Gel® Imager:
Revolutionizing Gel Documentation

E-Gel™ Imager Demo
Unboxing the
E-Gel
® Imager
Unboxing the E-Gel™ Imager

Ordering Information

Sku Name Size Price Qty
4466613 E-Gel® Imager System with E-Gel® Adaptor 1 each USD 5,890.00
4466612 E-Gel® Imager System with Blue Light Base 1 each USD 6,950.00
4466611 E-Gel® Imager System with UV Light Base 1 each USD 6,950.00
4466604 E-Gel® Imager Adaptor Base 1 each USD 473.00
4466603 E-Gel® Imager Blue Light Base 1 each USD 1,250.00
4466602 E-Gel® Imager UV Light Base 1 each USD 1,250.00
4466606 E-Gel® Imager Universal Filter 1 each USD 260.00
4466607 E-Gel® Imager Qdot® 625 Filter 1 each USD 260.00
4466608 E-Gel® Imager UV/SYBR® Filter 1 each USD 260.00
4466610 E-Gel® Imager Quantitation USB Dongle 1 each USD 295.00
4466605 E-Gel® Imager Band Excision Kit 1 each USD 310.00
4473061 E-Gel® Imager White-Light Conversion Screen 1 each USD 295.00
For Research Use Only. Not for human or animal therapeutic or diagnostic use.