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Obtaining high-quality, intact RNA is the first, and often most critical, step in performing gene expression analysis. In order to isolate high-quality RNA, the tissue has to be either: a) processed immediately after harvest, b) snap-frozen, or c) stabilized in an intermediary solution to preserve RNA integrity and allow for storage.  Life Technologies offers several products designed specifically to stabilize and preserve the integrity of RNA either at the point of collection or even post-collection:   RNAlater® Tissue Collection: RNA Stabilization Solution;  RNAlater®-ICE Frozen Tissue Transition Solution;  and THE RNA Storage Solution.

Product Selection Guide

Function Product Sample Types Description
RNAStabilization ProductsRNAlater® Tissue Collection: RNA Stabilization SolutionCells and tissuesAqueous, nontoxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA
RNAlater®-ICE Frozen Tissue Transition SolutionCells and tissuesPrevents RNA degradation during tissue thawing
THE RNA Storage SolutionPurified RNAMinimizes base hydrolysis by chelating free cations from solution with sodium citrate and by maintaining a low pH
Tempus® Blood RNA Tubes   BloodEvacuated blood tube designed to lyse and stabilize blood at the point of collection
LeukoLOCK™ Total RNA Isolation SystemBloodGlobin-free RNA from RNAlater®–stabilized leukocytes.   Anticoagulated blood is filter-fractionated, keeping only the leukocytes, and stabilized with RNAlater® solution for storage

Revolutionary reagent minimizes the need to immediately freeze samples to preserve RNA

Once saturated in RNAlater® solution, tissues can be removed, dissected, or weighed at room temperature, then returned to RNAlater® solution for continued storage. Tissues treated with RNAlater® solution and archived at –80°C can be subjected to 10 freeze/thaw cycles without affecting the quality of subsequently isolated RNA (Figure 1). Samples stored in RNAlater® solution can be shipped on wet ice or even at room temperature if shipped overnight. Figure 2 shows data from a ribonuclease protection assay using RNA from tissue stored in RNAlater® solution.

Learn More about RNAlater®

 Figure 1. RNA Stability of RNAlater®-Treated Tissues After Multiple Freeze/Thaw Cycles.
Mouse heart and liver were dissected and placed in RNAlater® solution. The tissues were frozen on dry ice and thawed at room temperature for the indicated number of cycles. RNA was isolated using RNAWIZ™, electrophoresed on a 1% formaldehyde/agarose gel, ethidium bromide stained, and photographed.

 

 Figure 2. Ribonuclease Protection Assay Using Mouse Tissues Stored in RNAlater® Solution.
Tissues were stored in RNAlater® solution for 1 or 4 weeks at 4°C. RNA was isolated from each tissue and analyzed using the RPA III™ Kit.

Ordering Information

Sku Name Size Price Qty
AM7020 RNAlater® Stabilization Solution 100 mL USD 116.00
AM7021 RNAlater® Stabilization Solution 500 mL USD 363.00
AM7024 RNAlater® Stabilization Solution 1 x 250 mL USD 257.00
AM7023 RNAlater® Stabilization Solution 20 x 5 mL USD 231.00
AM7022 RNAlater® Stabilization Solution 50 x 1.5 mL USD 219.00
AM7030 RNAlater®-ICE Frozen Tissue Transition Solution 25 mL USD 84.50
AM7000 THE RNA Storage Solution 10 x 1 mL USD 39.50
AM7001 THE RNA Storage Solution 50 mL USD 39.50
4342792 Tempus™ Blood RNA Tube 50 tubes USD 303.00
AM1923 LeukoLOCK™ Total RNA Isolation System 20 preps USD 670.00

RNA Stabilization Support Materials

TechNotes Articles

  • Make Time Stand Still with RNAlater® - TechNotes 10(1): The Ambion® RNAlater® Tissue Collection: RNA Stabilization Solution that makes it possible for researchers to postpone RNA isolation for days, weeks, or even months after tissue collection without sacrificing RNA integrity.
  • Thaw Frozen Tissues Without Damaging RNA - TechNotes 10(2): RNAlater®-ICE solution is a unique RNA-stabilizing solution—simply drop frozen tissues into RNAlater®-ICE solution and walk away! The tissues thaw without risk of RNA degradation. Once thawed the tissues can be processed like fresh tissues.

 

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