Biochemical Kinase Assays

Thermo Fisher Scientific offers you the broadest set of biochemical fluorescent assays to address high-throughput screening, hit confirmation and lead optimization programs.  All Life Technologies kinase assay technologies are homogenous, non-radioactive, and highly miniaturizable – and are available as kits, custom or bulk reagents.

For quality kinase profiling data on rapid timelines, choose SelectScreen®, the industry’s most utilized kinase profiling service.

To compile the components needed to make the right biochemical kinase assay for your research, use our new Drug Discovery Assay Maker™ web tool.

Request Assay Information        Use Assay Maker™

Choose the right biochemical HTS kinase assay for your research?

Compatible with fluorescently labeled whole protein substrates Ideal for kinetic studies including on/off rates or for kinases with no known substrate Validated specifically for lipid kinases; can use with unmodified peptide or lipid substrates Primary assay for SelectScreen® Kinase Profiling Service
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  LanthaScreen® Activity Assay LanthaScreen® Eu Kinase Binding Assay Adapta® Assay Z'-LYTE® Assay
Application Technology basis Phospho-antibody TR-FRET Tracer-based TR-FRET ADP detection by TR-FRET FRET peptide phosphorylation
Application Primary screening Unactivated or low-activity kinase target screening Primary and secondary screening Secondary screening, hit validation, and profiling
Number of kinases validated 200 over 320 20 233
Readout Endpoint Endpoint or Kinetic Endpoint Endpoint
Total assay volume 20 µL 15 µL 15 µL 20 µL
ATP concentration range Tested up to 1 mM ATP not used Tested from 10 to 100 µM Tested up to 1 mM
Substrate type Peptide or protein No substrate needed Peptide, protein or lipid Peptide
Total assay volume Substrate concentration range 100 nM - 1 µM Flexible; must be greater than ATP concentration 2 µM only
Plate signal stability Up to 18 hours Up to 24 hours Up to 18 hours 4 - 18 hours (kit-dependent)
Cell lysate compatible
Overcoming interference Time-resolved readout allows read after interfering signals have decayed Time-resolved readout allows read after interfering signals have decayed; use of epitope-tag ensures no contaminating kinase is detected Time-resolved readout allows read after interfering signals have decayed; red Alexa Fluor® dye as acceptor further enhances ability to overcome interference Preread at 445/520 nm
Cost per well $ - $$ $ - $$ $$ - $$$ $$
Total assay volume

Optimize with multiple high-throughput assay formats

Thermo Fisher Scientific offers four high-throughput assay technologies for your biochemical kinase research.

Lower Throughput Assay Technologies

  • Omnia® Kinase Assays - Use for mechanistic studies. Simple mix-and-read fluorescence-based assay allows measurements in real time (kinetics).
  • NDP Sensor - A universal, real-time kinetic kinase assay platform at a very low price per well