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Life Technologies offers you the broadest set of biochemical fluorescent assays to address high-throughput screening, hit confirmation and lead optimization programs.  All Life Technologies kinase assay technologies are homogenous, non-radioactive, and highly miniaturizable – and are available as kits, custom or bulk reagents.

For quality kinase profiling data on rapid timelines, choose SelectScreen®, the industry’s most utilized kinase profiling service.

To compile the components needed to make the right biochemical kinase assay for your research, use our new Drug Discovery Assay Maker™ web tool.

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Choose the right biochemical HTS kinase assay for your research

  Compatible with fluorescently labeled whole protein substrates Ideal for kinetic studies including on/off rates or for kinases with no known substrate Validated specifically for lipid kinases; can use with unmodified peptide or lipid substrates Primary assay for SelectScreen® Kinase Profiling Service
Technology
basis
Phospho-antibody TR-FRETTracer-based TR-FRETADP detection by TR-FRETFRET peptide phosphorylation


Application
Primary screeningUnactivated or low-activity kinase target screeningPrimary and secondary screeningSecondary screening, hit validation, and profiling
Number of kinases
validated
200 over 320 20 233
ReadoutEndpointEndpoint or KineticEndpointEndpoint
Total assay volume 20 µL15 µL 15 µL20 µL
ATP concentration range Tested up to 1 mM ATP not used Tested from 10 to 100 µM Tested up to 1 mM
Substrate type Peptide or protein No substrate needed Peptide, protein or lipid Peptide
Substrate concentration range 100 nM - 1 µM No substrate needed Flexible; must be greater than ATP concentration 2 µM only
Plate signal stability Up to 18 hours Up to 24 hours Up to 18 hours 4 - 18 hours (kit-dependent)
Cell lysate compatible yes
Overcoming interference Time-resolved readout allows read after interfering signals have decayed Time-resolved readout allows read after interfering signals have decayed; use of epitope-tag ensures no contaminating kinase is detected Time-resolved readout allows read after interfering signals have decayed; red Alexa Fluor® dye as acceptor further enhances ability to overcome interferencePreread at 445/520 nm
Cost per well $ - $$ $ - $$ $$ - $$$ $$
Use in SelectScreen® Kinase Profiling Services yes yes yes
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Optimize with multiple high-throughput assay formats

Life Technologies offers four high-throughput assay technologies for your biochemical kinase research.

Lower Throughput Assay Technologies

  • Omnia® Kinase Assays - Use for mechanistic studies. Simple mix-and-read fluorescence-based assay allows measurements in real time (kinetics).
  • NDP Sensor - A universal, real-time kinetic kinase assay platform at a very low price per well