Our LanthaScreen® kinase activity assay toolbox of "mix and match" assay reagents is designed to enable rapid assay configuration. Our portfolio of phospho-specific antibodies, matched fluorescent peptide substrates and in-house produced kinases have been tested in >200 different kinase assays to date.

Phospho-specific Antibodies with Peptide Substrates

To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities.  Matched and validated substrates are available for kinase assay configuration.

These antibodies may also be available with Europium labels, contact us at discoverysciences@invitrogen.com, for more information.

Tyrosine Kinase Assays

SKU Product Catalog Size
PV5692LanthaScreen® Eu-PY20 Antibody Kit25μg
PV5696LanthaScreen® Eu-PY20 Antibody Kit1mg
PV5693Alexa Fluor® 647-Poly GT
1 nmol
PV5836Alexa Fluor® 647-Poly GT10 nmol
PV3552LanthaScreen® Tb-PY20 Antibody Kit25μg
PV3553LanthaScreen® Tb-PY20 Antibody Kit1mg
PV3610Fluorescein-Poly GT, 30 μM1mL
PV3611Fluorescein-Poly GAT, 30 μM1mL

Serine/Threonine Kinase Assays

SKU Product Catalog Size
PV3560LanthaScreen® Tb-pSer (PKC) Antibody Kit25μg
PV3561LanthaScreen® Tb-pSer (PKC) Antibody Kit1mg
PV3506Fluorescein-PKC Substrate1mg
PV3562LanthaScreen® Tb-IKBa [pSer32] Antibody Kit25μg
PV3563LanthaScreen® Tb-IKBa [pSer32] Antibody Kit1mg
PV3507Fluorscein-IKK substrate1mg
PV3564LanthaScreen® Tb-pCrosstide Antibody Kit25μg
PV3565LanthaScreen® Tb-pCrosstide Antibody Kit1mg
PV3509Fluorescein-Crosstide Substrate1mg
PV3566LanthaScreen® Tb-CREB pSer133 Antibody Kit25μg
PV3508Fluorescein-CREBtide Substrate, 1 mg/mL1mg
PV4899LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit25μg
PV4900LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit1mg
PV4901Fluorescein-ERM (LRRKtide) Substrate1mg
PV5130LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit25μg
PV5131LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit1mg
PV5132Fluorescein-p53 [Ser15] Substrate1mg

Phospho-specific Antibodies with Physiological Protein Substrates

As an extension to our peptide-based substrates for LanthaScreen® kinase activity assays, we now offer physiological protein substrates that are directly labeled with a green fluorescent protein (GFP) acceptor fluorophore. Pairing the GFP-labeled substrate with a corresponding terbium (Tb)-labeled phosphospecific antibody allows detection of the phosphorylated product.

Ordering Information

SKU Product Catalog Size
PV4451LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit25μg
PV4452LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit1mg
PV4445GFP-ATF2 (19-96)20 nmol
PV4453LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit25μg
PV4454LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit1mg
PV4446GFP-c-jun (1-179)20 nmol
PV4757LanthaScreen® Tb-4E-BP1 [pThr46] Antibody Kit25μg
PV4758LanthaScreen® Tb-4E-BP1 (pThr46) Antibody Kit1mg
PV4759GFP-4E-BP120 nmol
PV4810LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit25μg
PV4811LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit1mg
PV4809GFP-eIF2a20 nmol
PV4813LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit25μg
PV4814LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit1mg
PV4812Fluorescein-MEK120 nmol
PV4844LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit25μg
PV4845LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit1mg
PV5211GFP-STAT120 nmol
PV4846LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit25μg
PV4847LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit1mg
PV5212GFP-STAT320 nmol

Buffers and Other Supplemental Assay Reagents

To complete our offering of antibodies and substrates for the development of TR-FRET kinase assays, we offer a selection of validated kinase buffers and other assay reagents.

Ordering Information

SKU Product Catalog Size
PV3574TR-FRET Dilution Buffer100mL
PV31895X Kinase Buffer A4mL
PV51255X Kinase Buffer Q4mL
PV51015X Kinase Buffer R4mL
PV5213 5X Kinase Buffer S4mL
PV47941X mTOR Assay Buffer20mL
P2825Kinase Quench Buffer1mL
PV322710 mM ATP500μL
P23251M DTT1mL