This table illustrates the reactivity of our Z´-LYTE™ Peptide Substrates using a broad range of kinases we have tested at Invitrogen. For each kinase in the left hand column, we list the Z´-LYTE™ substrate(s) that are recommended in the Z´-LYTE™ assay. We have substrates representing two classes of kinases, Tyrosine Kinase substrates and Serine/Theonine Kinase substrates. The number in the table refers to the substrate number of the Z’-LYTE™ Detection Assay Kit within each class of kinases.

Tyr Kinase Z'-LYTE™ Assay Kit Assay Protocol
ABL1 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ABL1 E255K Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ABL1 G250E Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ABL1 T315I Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ABL1 Y253F Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ABL2 (ARG) Z'LYTE™ Kit-Tyr 02 Peptide Protocol
AXL Z'LYTE™ Kit-Tyr 06 Peptide Protocol
BLK Z'LYTE™ Kit-Tyr 01 Peptide Protocol
BMX Z'LYTE™ Kit-Tyr 01 Peptide Protocol
BTK Z'LYTE™ Kit-Tyr 01 Peptide Protocol
CSF1R (FMS) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
CSK Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EGFR (ERBB1) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
EGFR (ERBB1) L858R Z'LYTE™ Kit-Tyr 04 Peptide Protocol
EGFR (ERBB1) L861Q Z'LYTE™ Kit-Tyr 04 Peptide Protocol
EGFR T790M Z'LYTE™ Kit-Tyr 04 Peptide Protocol
EGFR T790M L858R Z'LYTE™ Kit-Tyr 04 Peptide Protocol
EPHA1 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHA2 Z'LYTE™ Kit-Tyr 01 Peptide Protocol
EPHA4 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHA5 Z'LYTE™ Kit-Tyr 01 Peptide Protocol
EPHA8 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHB1 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHB2 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHB3 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
EPHB4 Z'LYTE™ Kit-Tyr 01 Peptide Protocol
ERBB2 (HER2) Z'LYTE™ Kit-Tyr 06 Peptide Protocol
ERBB4 (HER4) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
FER Z'LYTE™ Kit-Tyr 05 Peptide Protocol
FES (FPS) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
FGFR1 Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FGFR2 Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FGFR3 Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FGFR3 K650E Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FGFR4 Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FGR Z'LYTE™ Kit-Tyr 02 Peptide Protocol
FLT1 (VEGFR1) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FLT3 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
FLT3 D835Y Z'LYTE™ Kit-Tyr 02 Peptide Protocol
FLT4 (VEGFR3) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
FRK (PTK5) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
FYN Z'LYTE™ Kit-Tyr 02 Peptide Protocol
HCK Z'LYTE™ Kit-Tyr 02 Peptide Protocol
IGF1R Z'LYTE™ Kit-Tyr 01 Peptide Protocol
INSR Z'LYTE™ Kit-Tyr 01 Peptide Protocol
INSRR (IRR) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
ITK Z'LYTE™ Kit-Tyr 01 Peptide Protocol
JAK1 Z'LYTE™ Kit-Tyr 06 Peptide Protocol
JAK2 Z'LYTE™ Kit-Tyr 06 Peptide Protocol
JAK2 JH1 JH2 Z'LYTE™ Kit-Tyr 06 Peptide Protocol
JAK2 JH1 JH2 V617F Z'LYTE™ Kit-Tyr 06 Peptide Protocol
JAK3 Z'LYTE™ Kit-Tyr 06 Peptide Protocol
KDR (VEGFR2) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
KIT Z'LYTE™ Kit-Tyr 06 Peptide Protocol
KIT T670I Z'LYTE™ Kit-Tyr 06 Peptide Protocol
LCK Z'LYTE™ Kit-Tyr 02 Peptide Protocol
LTK (TYK1) Z'LYTE™ Kit-Tyr 06 Peptide Protocol
LYN A Z'LYTE™ Kit-Tyr 02 Peptide Protocol
LYN B Z'LYTE™ Kit-Tyr 02 Peptide Protocol
MATK (HYL) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
MERTK (CMER) Z'LYTE™ Kit-Tyr 02 Peptide Protocol
MET (CMET) Z'LYTE™ Kit-Tyr 06 Peptide Protocol
MET M1250T Z'LYTE™ Kit-Tyr 06 Peptide Protocol
MST1R (RON) Z'LYTE™ Kit-Tyr 06 Peptide Protocol
MUSK Z'LYTE™ Kit-Tyr 04 Peptide Protocol
NTRK1 (TRKA) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
NTRK2 (TRKB) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
NTRK3 (TRKC) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
PDGFRA (PDGFR ALPHA) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
PDGFRA D842V Z'LYTE™ Kit-Tyr 04 Peptide Protocol
PDGFRA T674I Z'LYTE™ Kit-Tyr 04 Peptide Protocol
PDGFRA V561D Z'LYTE™ Kit-Tyr 04 Peptide Protocol
PDGFRB (PDGFR BETA) Z'LYTE™ Kit-Tyr 04 Peptide Protocol
PTK2 (FAK) Z'LYTE™ Kit-Tyr 07 Peptide Protocol
PTK2B (FAK2) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
PTK6 (BRK) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
RET Z'LYTE™ Kit-Tyr 02 Peptide Protocol
RET V804L Z'LYTE™ Kit-Tyr 02 Peptide Protocol
RET Y791F Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ROS1 Z'LYTE™ Kit-Tyr 01 Peptide Protocol
SRC Z'LYTE™ Kit-Tyr 02 Peptide Protocol
SRC N1 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
SRMS (SRM) Z'LYTE™ Kit-Tyr 01 Peptide Protocol
SYK Z'LYTE™ Kit-Tyr 02 Peptide Protocol
TEK (TIE2) Z'LYTE™ Kit-Tyr 05 Peptide Protocol
TXK Z'LYTE™ Kit-Tyr 06 Peptide Protocol
TYK2 Z'LYTE™ Kit-Tyr 03 Peptide Protocol
TYRO3 (RSE) Z'LYTE™ Kit-Tyr 02 Peptide Protocol
YES1 Z'LYTE™ Kit-Tyr 02 Peptide Protocol
ZAP70 Z'LYTE™ Kit-Tyr 07 Peptide Protocol
Ser/Thr Kinase Z'-LYTE™ Assay Kit Assay Protocol
ACVR1B (ALK4) Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
ADRBK1 (GRK2) Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
ADRBK2 (GRK3) Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
AKT1 (PKB ALPHA) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
AKT2 (PKB BETA) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
AKT3 (PKB GAMMA) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
AMPK A1/B1/G1 Z'LYTE™ Kit-Ser/Thr 23 Peptide Protocol
AMPK A2/B1/G1 Z'LYTE™ Kit-Ser/Thr 23 Peptide Protocol
AURKA (AURORA A) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
AURKB (AURORA B) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
AURKC (AURORA C) Z'LYTE™ Kit-Ser/Thr 19 Peptide Protocol
BRAF Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
BRAF V559E Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
BRSK1 (SAD1) Z'LYTE™ Kit-Ser/Thr 21 Peptide Protocol
CAMK1D (CAMKI DELTA) Z'LYTE™ Kit-Ser/Thr 25 Peptide Protocol
CAMK2A (CAMKII ALPHA) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
CAMK2B (CAMKII BETA) Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
CAMK2D (CAMKII DELTA) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
CAMK4 (CAMKIV) Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
CDC42 BPB (MRCKB) Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
CDC42BPA (MRCKA) Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
CDK1/CYCLIN B Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
CDK2/CYCLIN A Z'LYTE™ Kit-Ser/Thr 12 Peptide Protocol
CDK5/P25 Z'LYTE™ Kit-Ser/Thr 12 Peptide Protocol
CDK5/P35 Z'LYTE™ Kit-Ser/Thr 12 Peptide Protocol
CHEK1 (CHK1) Z'LYTE™ Kit-Ser/Thr 19 Peptide Protocol
CHEK2 (CHK2) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
CLK1 Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
CLK2 Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
CLK3 Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
CSNK1A1 (CK1 ALPHA1) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
CSNK1D (CK1 DELTA) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
CSNK1E (CK1 EPSILON) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
CSNK1G1 (CK1 GAMMA 1) Z'LYTE™ Kit-Ser/Thr 05 Peptide Protocol
CSNK1G2 (CK1 GAMMA 2) Z'LYTE™ Kit-Ser/Thr 05 Peptide Protocol
CSNK1G3 (CK1 GAMMA 3) Z'LYTE™ Kit-Ser/Thr 05 Peptide Protocol
CSNK2A1 (CK2 ALPHA 1) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
CSNK2A2 (CK2 ALPHA 2) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
DAPK3 (ZIPK) Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
DCAMKL2 (DCK2) Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
DYRK1A Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
DYRK1B Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
DYRK3 Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
DYRK4 Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
EEF2K Z'LYTE™ Kit-Ser/Thr 24 Peptide Protocol
FRAP1 (MTOR) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
GRK4 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
GRK5 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
GRK6 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
GRK7 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
GSK3A Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
GSK3B (GSK3 BETA) Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
HIPK1 (MYAK) Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
HIPK2 Z'LYTE™ Kit-Ser/Thr 09 Peptide Protocol
HIPK3 (YAK1) Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
HIPK4 Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
IKBKB (IKK BETA) Z'LYTE™ Kit-Ser/Thr 05 Peptide Protocol
IKBKE (IKK EPSILON) Z'LYTE™ Kit-Ser/Thr 11 Peptide Protocol
IRAK4 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MAP2K1 (MEK1) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAP2K2 (MEK2) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAP2K6 (MKK6) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAP3K8 (COT) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAP3K9 (MLK1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MAP4K2 (GCK) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MAP4K4 (HGK) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MAP4K5 (KHS1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MAPK1 (ERK2) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAPK10 (JNK3) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPK11 (P38 BETA) Z'LYTE™ Kit-Ser/Thr 15 Peptide Protocol
MAPK12 (P38 GAMMA) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAPK13 (P38 DELTA) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAPK14 (P38 ALPHA) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPK3 (ERK1) Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
MAPK8 (JNK1) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPK9 (JNK2) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK2 Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK3 Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK5 (PRAK) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
MARK1 (MARK) Z'LYTE™ Kit-Ser/Thr 21 Peptide Protocol
MARK2 Z'LYTE™ Kit-Ser/Thr 21 Peptide Protocol
MARK3 Z'LYTE™ Kit-Ser/Thr 25 Peptide Protocol
MARK4 Z'LYTE™ Kit-Ser/Thr 25 Peptide Protocol
MELK Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
MINK1 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MKNK1 (MNK1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MST4 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
MYLK2 (SKMLCK) Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
NEK1 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
NEK2 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
NEK4 Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
NEK6 Z'LYTE™ Kit-Ser/Thr 22 Peptide Protocol
NEK7 Z'LYTE™ Kit-Ser/Thr 22 Peptide Protocol
NEK9 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PAK1 Z'LYTE™ Kit-Ser/Thr 19 Peptide Protocol
PAK2 (PAK65) Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
PAK3 Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
PAK4 Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
PAK6 Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
PAK7 (KIAA1264) Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
PASK Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PDK1 Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
PHKG1 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PHKG2 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PIM1 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PIM2 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PKN1 (PRK1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PLK1 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
PLK2 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
PLK3 Z'LYTE™ Kit-Ser/Thr 16 Peptide Protocol
PRKACA (PKA) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
PRKCB2 (PKC BETA II) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PRKCN (PKD3) Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
PRKCQ (PKC THETA) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
PRKD1 (PKC MU) Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
PRKD2 (PKD2) Z'LYTE™ Kit-Ser/Thr 17 Peptide Protocol
PRKG1 Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
PRKG2 (PKG2) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
PRKX Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
RAF1 (CRAF) Y340D Y341D Z'LYTE™ Kit-Ser/Thr 03 Peptide Protocol
ROCK1 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
ROCK2 Z'LYTE™ Kit-Ser/Thr 13 Peptide Protocol
RPS6KA1 (RSK1) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
RPS6KA2 (RSK3) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
RPS6KA3 (RSK2) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
RPS6KA4 (MSK2) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
RPS6KA5 (MSK1) Z'LYTE™ Kit-Ser/Thr 01 Peptide Protocol
RPS6KA6 (RSK4) Z'LYTE™ Kit-Ser/Thr 20 Peptide Protocol
RPS6KB1 (P70S6K) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
SGK (SGK1) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
SGK2 Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
SGK3 (SGKL) Z'LYTE™ Kit-Ser/Thr 06 Peptide Protocol
SNF1LK2 (QIK) Z'LYTE™ Kit-Ser/Thr 25 Peptide Protocol
SRPK1 Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
SRPK2 Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
STK22B (TSSK2) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
STK22D (TSSK1) Z'LYTE™ Kit-Ser/Thr 04 Peptide Protocol
STK23 (MSSK1) Z'LYTE™ Kit-Ser/Thr 18 Peptide Protocol
STK24 (MST3) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
STK25 (YSK1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
STK3 (MST2) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
STK4 (MST1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
TAOK2 (TAO1) Z'LYTE™ Kit-Ser/Thr 07 Peptide Protocol
TBK1 Z'LYTE™ Kit-Ser/Thr 05 Peptide Protocol