We have established a strictly controlled validation process to ensure target consistency and reproducibility. Each enzyme is:

  • Sequence validated
  • Expressed according to strictly controlled processes
  • Evaluated by SDS-PAGE
  • Quality controlled by mass spectrometry
  • Activity assessed in radiometric direct phosphorylation assay

Validated PKC

Figure 1. 
Sample validation data for PKC zeta

If we do not have the kinase you want, or you need other levels of validation to conform with your in-house processes, let us know.  Under our custom solutions program we can supply our targets or your targets to meet your validation needs.  Email us at discoveryservices@invitrogen.com for more information.