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TaqMan® MicroRNA Assays—Unrivaled sensitivity, specificity, and dynamic range
Our MicroRNA solutions feature TaqMan® MicroRNA Assay sensitivity, specificity, and dynamic range for unsurpassed miRNA quantitation. Our comprehensive Applied Biosystems solution provides the flexibility to meet different experimental needs and is supported by a complete range of reagents, assays, and real-time PCR instruments.

MicroRNA Assay & Array Workflows: Find the Products You Need for Every Step

Get Same-Day Results with Megaplex™ Primer Pools and TaqMan® MicroRNA Arrays

  • Megaplex™ Primer Pools simplify and accelerate miRNA profiling by reducing hands-on time and the amount of sample you need to as little as 1ng of total human, mouse or rat RNA—up to 100 times less material than existing analysis tools.
  • Matched with the streamlined workflow enabled by TaqMan® MicroRNA Arrays, you can achieve reproducible RT-PCR data that delivers unrivaled sensitivity and specificity across a comprehensive set of miRNA targets derived from the Sanger database.

Also Available in 384-well Arrays

TaqMan® MicroRNA Arrays provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured 384-well micro fluidic card, reducing experimental variability and the effort required to run 384 assays in parallel.

Assay Workflow

Step 1:
Extract Total RNA
Step 2:
Reverse Transcribe RNA
Step 3:
Set Up Singleplex TaqMan
® MicroRNA Assay Reactions
Step 4:
Perform Real-Time PCR Amplification
Step 5:
Analyze Data
  • RT-PCR Software
         
30 min 65 min 25 min 90 min

Array Workflow

Step 1:
Prepare Sample
Step 2:
Reverse Transcribe
Step 3:
Amplify cDNA (optional)
Step 4:
Dilute cDNA & Load TaqMan
® Array
Step 5:
Run TaqMan
® Array
Step 6:
Analyze Data
  • Diluted RT product
  • Fast Real-Time PCR System
  • RT-PCR Software
           
30 min 150 min 90 min 10 min 120 min