The Ambion® MessageAmp™ Kits are compatible with all major array platforms and types of labeling (i.e. biotin, amino allyl, Cy™dye). Kits for high throughput automation are also available. To choose the best kit for your needs, see the chart below. 

RNA Amplification Kit Selection Guide

Compatible Platforms:

Affymetrix®  and Illumina® Arrays

Agilent® Oligo Microarrays


Spotted Homebrew Arrays

Non-Platform Specific, Unlabeled aRNA

Type of Labeling:

Biotin Labeled aRNA

Amino Allyl-Cy™Dye Labeled aRNA

aRNA

Amino Allyl aRNA

RNA Input

Eukaryotic Total RNA

100 pg–20 ng — Two Rounds of Amplification

Down to 50 ng

MessageAmp™ II
P/N AM1751
MessageAmp™ II-Biotin
P/N AM1791

Enhanced Kits

Illumina® TotalPrep™ RNA
Amplification Kit

P/N AMIL1791

(for Illumina Sentrix® Arrays only)

Amino Allyl MessageAmp™ II Kit P/N AM1753
With Cy™3 P/N AM1795

With Cy™5 P/N AM1796
With Cy™3/Cy™5 Dyes
P/N AM1797

(Single or 2 round
amplification protocol)

MessageAmp™ II aRNA
Amplification Kit
(Single or 2 round
amplification protocol)

P/N AM1751

5X MessageAmp™ II
aRNA Amplification Kit

(Single or 2 round
amplification protocol)

P/N AM1751-5

Amino Allyl
MessageAmp™ II aRNA
Amplification Kit
(Single or 2 round
amplification protocol)

P/N AM1753

20 ng–500 ng — Single Round Amplification

35 ng–500 ng — Single Round Amplification

NEW! MessageAmp™ Premier RNA Amplification Kit
10 rxns P/N 4385821

30 rxns P/N AM1792

100 rxns P/N 4383452

NEW! MessageAmp™ III RNA
Amplification Kit

10 rxns P/N 4383451

30 rxns P/N AM1793

Illumina® TotalPrep™ RNA
Amplification Kit
(for Illumina Sentrix® Arrays only)

P/N AMIL1791

Amino Allyl MessageAmp™ II Kit P/N AM1753
With Cy™3 P/N AM1795

With Cy™5 P/N AM1796
With Cy™3/Cy™5 Dyes
P/N AM1797

(Single round amplification protocol)

MessageAmp™ II aRNA
Amplification Kit
(Single or 2 round
amplification protocol)

P/N AM1751

Amino Allyl
MessageAmp™ II aRNA
Amplification Kit
(Single round
amplification protocol)

P/N AM1753

96-well plates
20 ng–500 ng
per well

NEW! MessageAmp™ Premier RNA Amplification Kit
100 rxns P/N 4383452
and Biotinylated UTPs

Custom Kit Available
Please Inquire,
custom@appliedbiosystems.com

MessageAmp™ II-96
aRNA Amplification Kit (Single or 2 round

amplification protocol)

P/N AM1819

Amino Allyl
MessageAmp™ II-96 aRNA
Amplification Kit
(Single or 2 round
amplification protocol)

P/N AM1821

Bacterial RNA

Total RNA
100 ng–
1000 ng

Enriched RNA
10 ng–500 ng

MessageAmp™ II-Bacteria RNA Amplification Kit
P/N AM1790
and Biotinylated UTPs

Custom Kit Available
Please Inquire,
custom@appliedbiosystems.com

MessageAmp™ II-Bacteria
RNA Amplification Kit
P/N AM1790

MessageAmp™ II-Bacteria
RNA Amplification Kit
P/N AM1790
and
Ambion Amino
Allyl UTPs

Recommendations are based on the required aRNA yields for at least one microarray hybridization.

Exact yield will depend on total RNA input.

For Research Use Only. Not for use in diagnostic procedures.

Ordering Information

Sku Name Size Price Qty
AM1793 MessageAmp™ III RNA Amplification Kit 30 reactions USD 2,505.00
AM1792 MessageAmp™ Premier RNA Amplification Kit 30 reactions USD 2,493.75
AM1751 MessageAmp™ II aRNA Amplification Kit 20 reactions USD 1,260.00
AM1791 MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit 20 reactions USD 1,799.70
AM1797 Amino Allyl MessageAmp™ II aRNA Amplification Kit, with Cy™3/Cy™5 dyes (1:3) 20 reactions USD 1,768.20
AM1821 Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit 100 reactions USD 5,554.50