标准PCR酶

Taq DNA polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. It can withstand prolonged incubation at temperatures up to 95“C without significant loss of activity.

Life Technologies most popular Taq DNA polymerase for standard PCR amplification is AmpliTaq® DNA polymerase. For higher specificity we recommend one of our highly referenced hot start enzymes, AmpliTaq Gold® for templates under 3 kb and Platinum® Taq DNA Polymerase for templates 3-10 kb. AmpliTaq® polymerases are also available in 360 buffer formats, ultra-pure enzymes that help reduce false positive results and improve amplification of low-level target sequences. Many Life Technologies Taq Polymerases have optimized protocols on Veriti® thermal cyclers.

  • Amplification of a variety of PCR templates up to 10 kb in size
  • Options to use with GC-rich templates

Which Standard PCR Enzyme Is Right for You?

  Value Choice for non-Hot Start DNA Polymerase Value Choice for non-Hot Start DNA Polymerase General Choice for Hot Start DNA Polymerase Premium Choice for Hot Start DNA Polymerase
Top Selleryesyes
Improved Specificityyesyes
Improved Sensitivityyes
Amplicon Size Up to 5 kb Up to 5 kb Up to 5 kb Up to 10 kb
Improved Yield yes
GC-Rich Formatyes yes
Activation Time Immediate Immediate 10 minutes 30 seconds
Master Mix/Super Mix Format yes yesyesyes
Price/Reaction$0.30 - $0.55 $0.44 - $0.55 $0.69
(4,000 Reactions)
$0.72
(5,000 Reactions)
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