Platinum® Taq DNA Polymerase is a hot-start enzyme that has been trusted by researchers for robust, reliable amplification for years. This antibody-mediated, hot-start product provides high specificity for use in everyday PCR applications ranging from cloning to genotyping to mutagenesis.

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Platinum® Taq Technology

Platinum® Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. Due to specific binding of the inhibitor, Platinum® Taq DNA Polymerase is provided in an inactive form. The DNA polymerase is then activated in a temperature-dependent manner (at 94ºC) during the start of PCR. Once dissociated from the inhibitor, the Taq DNA polymerase regains its full activity.

More Versatility for PCR products up to 10 kb

Use Platinum® Taq DNA Polymerase for expected PCR products up to 10 kb.  The optional KB Extender gives more versatility to the Platinum® Taq DNA polymerase in PCR assays. 

PCR Products

Panel A.  A 4.1-kb region of genomic target was amplified from 200-400 ng K562 genomic DNA without KB Extender (Lanes 1-2), with KB Extender at 2% final concentration (Lanes 3-4) using Platinum® Taq DNA Polymerase; the same products amplified with Competitor DNA Polymerase (Lanes 6-7).  Each reaction was performed in duplicate.

Panel B.  A 10-kb region of genomic target was amplified from 200-400 ng K562 genomic DNA without KB Extender (Lanes 1-2), with KB Extender at 2% final concentration (Lanes 3-4) using Platinum® Taq DNA Polymerase; the same products amplified with Competitor DNA Polymerase (Lanes 6-7).  Each reaction was performed in duplicate. 

Broader Magnesium Range

A 2.8-kb region of the human β-globin gene was amplified from 100 ng of human genomic DNA.

Using human genomic DNA, the amplification of a 2.8 kb region was evaluated at 1.0, 1.4, 1.8, 2.2, 2.6, and 3.0 mM magnesium (Lanes 1-6, respectively).

  • Panel A: Taq DNA Polymerase with room temperature assembly.

  • Panel B: Taq DNA Polymerase with assembly on ice and placing in a preheated (80ºC) thermal cycler.

  • Panel C: Platinum® Taq DNA Polymerase with room temperature assembly.
 

Improved specificity with Platinum® Taq DNA Polymerase

 

One unit of Platinum® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 min at 74ºC.

Panel A:  Detection of cloned HIV DNA in human genomic DNA. 1,000 copies of plasmid DNA with the HIV gag region was mixed with 100 ng of genomic DNA and amplified with primers SK38 and SK39.

  • Lane 1. Taq DNA Polymerase with room temperature assembly
  • Lane 2. Taq DNA Polymerase with manual hot start by addition of enzyme at 94ºC
  • Lane 3. Platinum® Taq DNA Polymerase with room temperature assembly

Panel B:  Amplification of 4.1 kb of human ß-globin from 100 ng of human genomic DNA.

  • Lane 1. Taq DNA Polymerase with room temperature assembly
  • Lane 2. Taq DNA Polymerase with assembly on ice and placing in a preheated (80ºC) thermal cycler
  • Lane 3. Platinum® Taq DNA Polymerase with room temperature assembly.temperature assembly

More Coverage for More Amplicons

Products amplified with Platinum® Taq DNA Polymerase (A); the same products amplified with Competitor DNA Polymerase (B). PCR reactions were run using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate.

     A. Platinum® Taq DNA Polymerase               B. Competitor DNA Polymerase

Platinum® Taq DNA Polymerase

High Specificity and Yield Comparison of Platinum® Taq DNA Polymerase to Competitor Polymerase

The data show a summary of two replicates for each of 40 amplicons, indicating the average specific yield for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).

The data show a summary of two replicates for each of 40 amplicons, indicating the average specificity for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).

Which Platinum® Taq DNA Polymerase is right for you?

  Platinum® Taq DNA Polymerase Platinum® Taq DNA Polymerase High Fidelity Platinum® Pfx DNA Polymerase Platinum® GenoType Tsp DNA Polymerase Platinum® Multiplex PCR Master Mix
Fidelity vs Taq 1x 6x 26x 1x 1x
Amplicon size 10 kb 20 kb 20 kb 500 bp 2.5 kb
Applications
Routine PCR        
High fidelity      
High specificity        
High yield      
Fast        
Long amplicon        
GC-rich targets        
PCR genotyping        
Multiplex          
Master mix available      
Direct gel loading available        

Use promo codes: US P1074284 and CA P1074285 when ordering and you will automatically receive the discount at time of purchase.

*Terms and conditions apply

For the reagent rewards, please go to lifetechnologies.com/rewards

Ordering Information

Sku Name Size Price Qty
11708013 Platinum® Pfx DNA Polymerase 100 reactions USD 131.00
11708021 Platinum® Pfx DNA Polymerase 250 reactions USD 314.00
11708039 Platinum® Pfx DNA Polymerase 500 reactions USD 546.00
10966018 Platinum® Taq DNA Polymerase 120 reactions USD 99.00
10966026 Platinum® Taq DNA Polymerase 300 reactions USD 258.00
10966034 Platinum® Taq DNA Polymerase 600 reactions USD 459.00
10966083 Platinum® Taq DNA Polymerase 5,000 reactions USD 3,820.00
11304011 Platinum® Taq DNA Polymerase High Fidelity 100 reactions USD 165.00
11304029 Platinum® Taq DNA Polymerase High Fidelity 500 reactions USD 770.00
11304102 Platinum® Taq DNA Polymerase High Fidelity 5,000 reactions USD 6,240.00
11509015 Platinum® Taq PCRx DNA Polymerase 500 reactions USD 628.00
73684050 Platinum® Tfi DNA Polymerase 500 units USD 412.00
60684050 Platinum® Tfi Exo(-) DNA Polymerase 500 units USD 427.00
12580015 Platinum® Blue PCR SuperMix 100 reactions USD 152.00
12580023 Platinum® Blue PCR SuperMix 1,000 reactions USD 1,316.00
12580049 Platinum® Blue PCR SuperMix 96, non-skirted 5 plates USD 858.00
12580031 Platinum® Blue PCR SuperMix 96, skirted 5 plates USD 848.00
11448024 Platinum® GenoType Tsp DNA Polymerase 250 units USD 349.00
11448032 Platinum® GenoType Tsp DNA Polymerase 2500 units USD 2,525.00
11306016 Platinum® PCR SuperMix 100 reactions USD 140.00
11306081 Platinum® PCR SuperMix 5,000 reactions USD 6,290.00
11306073 Platinum® PCR SuperMix 96, non-skirted 480 reactions USD 822.00
12532016 Platinum® PCR SuperMix High Fidelity 100 reactions USD 237.00
12532024 Platinum® PCR SuperMix High Fidelity 5,000 reactions USD 8,770.00
*Terms and Conditions:

This promotion is open to customers in the US and Canada. Discount will apply to orders received by Life Technologies no later than July 31, 2014. Discount is valid only for the following SKUs: 10966018, 10966026, 10966034 and 10966083. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.