TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.
- Highly specific – quantitiate miRNA transcription from a single genomic locus
- Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
- A new perspective – measure miRNA expression at the gene level
TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.
Design and Selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.
|Catalog #||Size||# of Reactions/Concentration|
|Pre-formulated assay (20x or 60x mix):||
|Documentation:||On compact disc:
|Assay Information File:||Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context sequence, Amplicon size)|
Shipping and Storage
Shipped at ambient temperature.
Store at –15ºC to –25ºC.
White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability
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