cDNA Synthesis with SuperScript® III RT
Efficient cDNA synthesis can help ensure reliable data in any downstream application, and synthesis of full-length cDNA eliminates the gene bias seen with many RT enzymes. Trusting your reverse transcription reactions to a SuperScript® III First Strand Synthesis Kit reduces false negative results with higher yields of full-length cDNA and more complete gene representation.
Choice of Kit Formats to Meet Your Needs
- SuperScript® III First Strand SuperMix for qRT-PCR contains reaction buffer, MgCl2, dNTPs and a 1:1 mixture of random hexamers and oligo dT primers all in one tube. Just add the SuperScript® III enzyme blend and your RNA.
- SuperScript® III First Strand SuperMix contains the same components listed above, but with random hexamers and oligo dT primers in separate tubes to suit any priming strategy.
- SuperScript® III First Strand Synthesis System for RT-PCR contains all the necessary components in separate tubes to allow full optimization of your RT reactions.