Perform both cDNA synthesis and PCR amplification in a convenient one-step formulation. Our One-Step RT-PCR Kits contain dNTPs and a mixture of reverse transcriptase and thermostable hot-start DNA polymerase in an optimized reaction buffer. Just add your RNA sample and gene-specific primers, and start your reactions.
What is the difference between one-step and two-step RT-PCR?
Which one-step RT-PCR kit is right for you?
|Standard choice for RT-PCR||Preferred choice for RT-PCR||Preferred choice for RT-PCR with high fidelity|
|Order now||Order now||Order now|
|SuperScript® One-Step RT-PCR System with Platinum® Taq DNA polymerase||SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA polymerase||SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity|
|Reverse transcriptase||SuperScript® II||SuperScript® III||SuperScript® III|
|Optimal reaction temp.||42°C||55°C||55°C|
|Max cDNA length||3 kb||4.5 kb||10 kb|
|Sensitivity||10 pg–1 µg Total RNA||0.01 pg–1 µg Total RNA||1 pg -1 ug Total RNA|
|PCR enzyme||Platinum® Taq DNA polymerase||Platinum® Taq DNA polymerase||Platinum® Taq High Fidelity DNA polymerase|