The Thermo Scientific™ HeavyPeptide IGNIS Prime Custom Peptide Quantitation Kit enables absolute quantitation of target peptides by mass spectrometry (MS) in less time than conventional methods of isotopically-labeled standard-based peptide quantitation. Upon tryptic digestion, the custom isotopically-labeled peptide yields 2 peptides of different masses that exhibit a 1:1 stoichiometry. Each dipeptide comes with controls for single-injection instrument calibration and peptide quantitation.

  • Rapid—decreases time to generate concentration curve (single vs. multiple runs by other methods)
  • Sensitive—enables the absolute quantitation of low-abundance proteins (attomolar sensitivity)
  • Precise—single-injection dilution curve removes LOD/LOQ bias
  • Flexible—multiple isotopic amino acids and reporter sizes available

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Specifications of Thermo Scientific HeavyPeptide IGNIS Prime Peptides

Dipeptide purity >95%†
Isotopic purity >99%
Size 10 X 1.0 μg (~0.5 nmol§)
Custom peptide length
  • Up to 20 amino acids in length
  • C-terminus must be arginine or lysine
  • Sequence must include a heavy amino acid
  • 10 X Isotopologue Dilution Curve Mixture (iDCM-8)
  • 10 X Isotopologue Bracketing Mixture (iBM-4)
  • 10 X 1 mL Reconstitution Solvent
†As measured by analytical reverse-phase HPLC
§The quantity provided is the gross weight of peptide, not the net peptide content, and is based on an average peptide content of 60% (corresponding to approximately 0.5 nmol).
  • Selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays
  • Biomarker discovery, verification and validation
  • Functional quantitative proteomics
  • Confirmation of results from RNA interference (RNAi)
  • Pharmacokinetics
  • Metabolomics
  • Clinical biochemistry for drug and metabolite monitoring
  • Anti-doping testing
  • Protein expression monitoring
  • Cell signal profiling and pathway validation 
How to build Thermo Scientific HeavyPeptide IGNIS Prime Peptides
Steps 1-3 in building Thermo Scientific HeavyPeptide IGNIS Prime Peptides
HeavyPeptide IGNIS isotopologues:
same sequence but variable mass
Isotopologue Sequence Δm§
HeavyPeptide IGNIS isotopologue iA AALPAAFK +6
HeavyPeptide IGNIS isotopologue iB AALPAAFK +11
HeavyPeptide IGNIS isotopologue iC AALPAAFK
HeavyPeptide IGNIS isotopologue iD AALPAAFK +16
HeavyPeptide IGNIS isotopologue iE AALPAAFK +21
HeavyPeptide IGNIS isotopologue iF AALPAAFK +26
HeavyPeptide IGNIS isotopologue iG AALPAAFK +31
HeavyPeptide IGNIS isotopologue iH AALPAAFK +36
HeavyPeptide IGNIS isotopologue iJ AALPAAFK +41
HeavyPeptide IGNIS isotopologue iK AALPAAFK +47
Steps 4 and 5 in building Thermo Scientific HeavyPeptide IGNIS Prime Peptides
† Isotopologue availability depends on the number of peptides to be quantitated (single peptide or multiplex).
‡ Red letters indicate isotopically labeled amino acids.
§ Compared to the iC isotopologue

Single-injection instrument calibration with Thermo Scientific Isotopologue Dilution Curve Mixture (iDCM-8).


Single-peptide and multiplex quantitation using the Thermo Scientific HeavyPeptide IGNIS Prime Peptide Quantitation Kit


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