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Let us answer your questions about which ELISA kit is right for your research. Whether you want to optimize your own assay or prefer a pre-optimized, ready-to-use kit for the fastest time to answer, we have an option for your research needs.

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Featured Guide to ELISA Kit Sizes and Format categories

Antibody Pair Kits

Matched, pre-titered, and fully optimized capture (coating) and detection antibodies allow you to build your own immunoassay.

Primary Antibodies

Search for primary antibodies against a wide range of markers, including signaling proteins, cell and organelle markers, cell junctions CD markers, and more.

Luminex® Assays

Bead-based technology enables the analysis of multiple targets in a single sample from a wide range of biological sources. Check our custom Luminex® assay panel.

Secondary Antibodies

Browse our secondary antibodies, with conjugates that include Alexa Fluor® and classic fluorescent dyes, HRP and AP, and more from a wide range of hosts.

Guide to ELISA kit sizes and formats

ELISA Kits (with pre-coated plate) are complete, ready-to-use kits that include a detailed protocol and all the required reagents to perform a quantitative sandwich ELISA with a variety of samples (e.g. serum, plasma, cell culture supernatants). Each kit has lot-optimized reagents titrated to the pre-coated plate to ensure maximum sensitivity and ease-of-use. Any changes to the protocol may affect the ELISA results and will require additional optimization to yield the best results.

Antibody Pair Kits include validated matched pairs of antibodies and calibrated standard for custom ELISA development. Using the recommended buffers described in the detailed protocol, these kits provide bulk antibody pairs for analyzing five to forty 96-well plates. The reagents are optimized for reliable performance using the specific antibody concentrations described in the protocol, but researchers can modify and re-optimize the protocol as needed.

Reagent Kits include pre-titered coating and detection antibodies, recommended buffers and a detailed protocol for preparing a quantitative sandwich ELISA. They require users to separately purchase plates and buffers and to coat the capture antibody at the specified concentration. Reagent Kits are designed to give optimal results for cell culture supernatants with the specific protocol. However, the reagent format provides flexibility to researchers to potentially modify and re-optimize the protocol as needed.

ELISA kit package types.

For most targets, the detection antibody is biotinylated, in which case the HRP Conjugate is streptavidin-HRP.

Kit Contents Antibody
Pair Kits
Reagent
Kits
Precoated
Plate Kits
Coating Antibody X X Coated
Detection Antibody X X X
Calibrated Standard X X X
HRP Conjugate   X X
TMB Substrate Solution   X X
Stop Solution   X X
Standard-Sample Diluent     X
Wash Buffer     X
HRP Dilution Buffer     X
Adhesive Plate Covers     X
Precoated 96-well Strip Plate     X
Package Sizes Sufficient For: 40
plates
5
plates
1, 2 or 5
plates
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Pierce Protein Methods Library
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