In-Cell ELISA Kits

While traditional Western blot analysis is time consuming and only semi-quantitative, the in-cell ELISA is an accurate method to determine the relative protein levels and degree of post-translational modification (PTM) among various cell types. The in-cell ELISA may be performed in 96- or 384-well microplates, are scalable and conserve cell culture and treatment reagents. The assay results are readily available for analysis because assay data is measured using a standard ELISA plate reader or infrared imaging system. Also, the assay is amenable to automation, which is ideal for siRNA studies and drug screens.

Popular In-Cell ELISA kit products

In-Cell ELISA Colorimetric Detection Kit
In-Cell ELISA Near Infrared Detection Kit
EGFR Colorimetric In-Cell ELISA Kit, Multispecies

Measure relative protein expression and modification levels in whole cells

Our in-cell ELISA colorimetric and near infrared detection kits are simple and convenient methods for the quantification of intracellular protein levels in whole cells.

Features of in-cell ELISA kits:

  • Two detection formats available
    • Colorimetric – compatible with standard ELISA plate readers
    • Near Infrared – compatible with the LI-COR Odyssey Infrared Imaging System
  • Simple, fast assay format
  • Ability to perform multiplex experiments
  • No special sample preparation required
  • Higher throughput compared with Western blotting
  • Cost efficient (one well vs. one lane) compared to Western blotting

Applications of in-cell ELISA kits:

  • High throughput determination of relative protein expression levels
  • Monitor dose-dependent post-translational protein modification
  • Multiplex analysis of targets in multiple cell lines
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Schematic overview comparing the Near IR and Colorimetric detection methods of the Thermo Scientific In-Cell ELISA Kits. The In-Cell ELISA Near Infrared Detection Kit allows two targets to be analyzed in the same well using two primary antibodies and detection with DyLight 680 and DyLight 800 conjugated secondary antibodies. The In-Cell ELISA Colorimetric Detection Kit allows multiple targets to be analyzed using primary antibodies in different wells detected with a horseradish peroxidase conjugate normalized to cell number with the whole-cell stain, Janus Green.

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Near IR and Colorimetric In-Cell ELISA Kits produce similar results. The Thermo Scientific In-Cell ELISA Near Infrared Detection Kit and the Thermo Scientific In-Cell ELISA Colorimetric Detection Kit were compared using EGFR activation in A431 cells as the model system. Similar fold-induction (see graph) and Z-factor (0.82 and 0.80, respectively) indicate comparable and robust performance of both kits.

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Near IR and Colorimetric In-Cell ELISA Kits produce similar results. The Thermo Scientific In-Cell ELISA Near Infrared Detection Kit and the Thermo Scientific In-Cell ELISA Colorimetric Detection Kit were compared using EGFR activation in A431 cells as the model system. Similar fold-induction (see graph) and Z-factor (0.82 and 0.80, respectively) indicate comparable and robust performance of both kits.


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Multiplex analysis of multiple targets using the In-Cell ELISA Near Infrared Detection Kit. Levels of total and phosphorlylated STAT3, STAT6 and EGFR were measured in untreated A431 cells and compared to cells treated with 100ng/mL EGF or both 100ng/mL EGF and 25 nM PD168393 (panel A). Graphing the data shows inhibition but not complete blocking of phosphorylation by 25nM PD168393 (panel B).

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